| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3391 | Thermotoga | TCCTGGATATTGCGACGGC | GCTCTTTCGCTTCGGTGGT | 59.93 | 60.67 | 275 | 39 |
100.00%
|
100.00%
|
| Primer 3392 | Thermotoga | TCTGAATCCAACGATCGTGGA | TCGGATGCGCTCTTTGCT | 59.18 | 59.73 | 231 | 39 |
100.00%
|
40.00%
|
| Primer 3393 | Thermotoga | CAACGCCACAAAACTCGCT | TCCGGTGGATGGGCAAAA | 59.64 | 59.15 | 299 | 39 |
100.00%
|
60.00%
|
| Primer 3394 | Thermotoga | TGGGTGGGTACATGGGGAA | ACGTGCAAGCCTCACCAAT | 60.15 | 60.23 | 299 | 39 |
100.00%
|
40.00%
|
| Primer 3395 | Thermotoga | ACACGGTCCAAAGGCTCTG | CGGGAAATCGCCTTCTGGT | 59.93 | 60.08 | 225 | 39 |
100.00%
|
80.00%
|
| Primer 3396 | Thermotoga | CAGACACGGTCCAAAGGCT | CGGGAAATCGCCTTCTGGT | 59.93 | 60.08 | 228 | 39 |
100.00%
|
80.00%
|
| Primer 3397 | Thermotoga | AGCACCATTGTGACCGCT | TGCCGTTTGTGGTCGTGA | 59.57 | 59.81 | 214 | 39 |
100.00%
|
80.00%
|
| Primer 3398 | Thermotoga | GATTCTGGAAGCGCTGGGT | GCCGAACTCCGAACCTTCA | 60.08 | 60.01 | 232 | 39 |
100.00%
|
40.00%
|
| Primer 3399 | Thermotoga | ATTCTGGAAGCGCTGGGT | GCCGAACTCCGAACCTTCA | 58.92 | 60.01 | 231 | 39 |
100.00%
|
40.00%
|
| Primer 3400 | Thermotoga | AAGGTTCTTCGCGTGGCT | TCCCCAAAAGCCAGCCAT | 59.57 | 59.14 | 193 | 39 |
100.00%
|
60.00%
|