| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 331 | Thermotoga | TGAAGCAGAGCAGAGGGGA | ACTGTGCGACGGTGTTGT | 60.23 | 59.81 | 200 | 118 |
100.00%
|
80.00%
|
| Primer 332 | Thermotoga | ATGTCACGCGAGATCCAGG | GGTGCTCTGTAACCTGACCC | 59.56 | 60.04 | 232 | 118 |
100.00%
|
40.00%
|
| Primer 333 | Thermotoga | TCACGCGAGATCCAGGAAC | GGTGCTCTGTAACCTGACCC | 59.49 | 60.04 | 229 | 118 |
100.00%
|
40.00%
|
| Primer 334 | Thermotoga | TGTCACGCGAGATCCAGGA | GGTGCTCTGTAACCTGACCC | 60.68 | 60.04 | 231 | 118 |
100.00%
|
40.00%
|
| Primer 335 | Thermotoga | ACGCGAGATCCAGGAACTC | GGTGCTCTGTAACCTGACCC | 59.19 | 60.04 | 227 | 118 |
100.00%
|
40.00%
|
| Primer 336 | Thermotoga | TGAAGCAGAGCAGAGGGGA | ACTGTGCGACGGTGTTGT | 60.23 | 59.81 | 200 | 118 |
100.00%
|
80.00%
|
| Primer 337 | Thermotoga | CCGTCACCACCTTCACGAT | TTTGCGACAACCTCCGGA | 59.71 | 59.18 | 226 | 118 |
100.00%
|
60.00%
|
| Primer 338 | Thermotoga | ACCTTCACGATAGCGGTGG | TTTGCGACAACCTCCGGA | 59.48 | 59.18 | 218 | 118 |
100.00%
|
60.00%
|
| Primer 339 | Thermotoga | TCGCGTCAGAGAAAATGGGA | TCGTCGATCTCCTCCAGCT | 59.39 | 59.78 | 211 | 117 |
100.00%
|
80.00%
|
| Primer 340 | Thermotoga | TCGCGTCAGAGAAAATGGGAA | TCGTCGATCTCCTCCAGCT | 60.00 | 59.78 | 211 | 117 |
100.00%
|
80.00%
|