| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 351 | Thermotoga | TTTCTGGCCTTGCGTGGT | ATTCCGAGTCCAAGGCCAG | 59.81 | 59.40 | 175 | 117 |
100.00%
|
80.00%
|
| Primer 352 | Thermotoga | ACTGGCCACGGTGATTCTG | CGTCAGCAGTCCAAGAGCT | 60.00 | 59.71 | 151 | 117 |
100.00%
|
100.00%
|
| Primer 353 | Thermotoga | ACGCTGCCATGGAAAAGGT | AGTTCACGCCACCGACAA | 60.23 | 59.50 | 189 | 117 |
100.00%
|
80.00%
|
| Primer 354 | Thermotoga | TGCCAGCGGTTCTCACAA | ACGCTTCCACGAGTCGAA | 59.49 | 58.96 | 291 | 117 |
100.00%
|
80.00%
|
| Primer 355 | Thermotoga | ACTCATCGGCAAGGCGAT | TGCCATTGCGGAAAGGGT | 59.10 | 59.88 | 205 | 117 |
100.00%
|
80.00%
|
| Primer 356 | Thermotoga | TACTACGGTGGAGGGCACA | TGCCATTGCGGAAAGGGT | 59.92 | 59.88 | 231 | 117 |
100.00%
|
80.00%
|
| Primer 357 | Thermotoga | AGGAACTCATCGGCAAGGC | TGCCATTGCGGAAAGGGT | 60.08 | 59.88 | 209 | 117 |
100.00%
|
80.00%
|
| Primer 358 | Thermotoga | TGCTGCCTTTGACGCACT | AAGCCCATCAGCGAGGAA | 60.20 | 58.60 | 202 | 117 |
100.00%
|
60.00%
|
| Primer 359 | Thermotoga | TTGCCCAGCTCAGCGAAT | TGCAGCGATGATCTCCGTC | 59.65 | 59.93 | 272 | 117 |
100.00%
|
40.00%
|
| Primer 360 | Thermotoga | CCTGGATATTGCGACGGCT | AGCTCTTTCGCTTCGGTGG | 59.93 | 60.37 | 275 | 117 |
100.00%
|
100.00%
|