| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3601 | Thermotoga | CGCTGTCACAGTTGGCAA | GGGACGGCTGTTCTGAAGT | 58.58 | 59.63 | 159 | 30 |
100.00%
|
80.00%
|
| Primer 3602 | Thermotoga | TCCGTTTCCTGCACGACA | GCGCACCCTGAACGTCTAT | 59.18 | 60.15 | 220 | 30 |
100.00%
|
80.00%
|
| Primer 3603 | Thermotoga | TCCTGACGGTGACCCTCAA | TTCCCCATGTACCCACCCA | 60.15 | 60.15 | 199 | 30 |
100.00%
|
80.00%
|
| Primer 3604 | Thermotoga | AGCGGTGGTGAGCTTCAA | TCAGCAAATCGCAGAGCCA | 59.17 | 60.00 | 200 | 30 |
100.00%
|
40.00%
|
| Primer 3605 | Thermotoga | TGGCACACCGGAAGCTAT | ACACGCAAGACAGCCCAT | 58.60 | 59.57 | 196 | 30 |
100.00%
|
60.00%
|
| Primer 3606 | Thermotoga | CCAGGTACCCAGAAACGGT | TCGCGACAGCCGATTTCA | 58.94 | 59.74 | 245 | 28 |
100.00%
|
80.00%
|
| Primer 3607 | Thermotoga | TGATGGAAGCGGAACTCGG | CGTTCCTCGAAAGCCAGGT | 59.78 | 60.00 | 165 | 28 |
100.00%
|
60.00%
|
| Primer 3608 | Thermotoga | ACGTGTTGAGTGGTGGCT | ACCAAGCCATGCCGTCAA | 59.09 | 59.88 | 293 | 28 |
100.00%
|
40.00%
|
| Primer 3609 | Thermotoga | TGGGCAAGTGCGAGTACAG | AGCTGTTACCGCACCGAA | 60.01 | 59.26 | 249 | 28 |
100.00%
|
100.00%
|
| Primer 3610 | Thermotoga | TTGTTCCGATCGGCAGGT | ACCACCACCGTTATCGAGC | 58.93 | 59.78 | 289 | 28 |
100.00%
|
80.00%
|