| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3621 | Thermotoga | ATGGGACTTCCGCTGGAGA | TTCGAGGAAACCTGCGCT | 60.31 | 59.26 | 297 | 25 |
100.00%
|
80.00%
|
| Primer 3622 | Thermotoga | AGGCACTTTTCGATGCTCAGA | TATGGCCTCCCGTCCTCTT | 60.00 | 59.69 | 284 | 25 |
100.00%
|
80.00%
|
| Primer 3623 | Thermotoga | CCAACGTAGCGGAAGTGGT | TGCCACCGGATCGTAGAGA | 60.01 | 60.08 | 299 | 25 |
100.00%
|
80.00%
|
| Primer 3624 | Thermotoga | AAGGGATCGCACACGTGA | TCCATCCACTCTGGCCAGA | 58.94 | 59.92 | 221 | 25 |
100.00%
|
80.00%
|
| Primer 3625 | Thermotoga | ACTTGCCGACGCGTTTGA | GGAGGCACTTTGTGTTCGC | 60.59 | 59.72 | 183 | 25 |
100.00%
|
40.00%
|
| Primer 3626 | Thermotoga | TGCACATCGGTGGAAGCT | TAAACCGGTGTCTCCTGCG | 59.25 | 59.71 | 170 | 24 |
100.00%
|
80.00%
|
| Primer 3627 | Thermotoga | TGCTGCTCTGAAGAACCCG | ACTTCCTCACGTGGTGTCG | 60.00 | 59.64 | 233 | 24 |
100.00%
|
80.00%
|
| Primer 3628 | Thermotoga | GCAAGCGGAAGAATGCTCG | AAGCCTGCCAGTGAACGT | 59.94 | 59.49 | 196 | 23 |
100.00%
|
40.00%
|
| Primer 3629 | Thermotoga | TCCGCTTGTGGTTTCCGT | CACAACCACGTCCCTTCCA | 59.49 | 59.85 | 172 | 23 |
100.00%
|
40.00%
|
| Primer 3630 | Thermotoga | TCCGCTTGTGGTTTCCGT | ACAACCACGTCCCTTCCAC | 59.49 | 59.85 | 171 | 23 |
100.00%
|
40.00%
|