| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 411 | Thermotoga | TCGACGAAGCAACGGACA | TTGCATGTTCGCTTCCGC | 59.28 | 59.44 | 254 | 114 |
100.00%
|
80.00%
|
| Primer 412 | Thermotoga | TCTCCTACATGGACAGCGC | TCACGCCTCCTCCACTGTA | 59.18 | 59.92 | 173 | 114 |
100.00%
|
80.00%
|
| Primer 413 | Thermotoga | CATCTCGACCACCAGCAAGA | TCACGCCTCCTCCACTGTA | 59.75 | 59.92 | 220 | 114 |
100.00%
|
80.00%
|
| Primer 414 | Thermotoga | TGCCAGCGGTTCTCACAA | GCTGCCACTTCCGGATCTT | 59.49 | 60.08 | 235 | 114 |
100.00%
|
80.00%
|
| Primer 415 | Thermotoga | CGACGGCTTACACAAACGC | AGCTCTTTCGCTTCGGTGG | 60.15 | 60.37 | 264 | 114 |
100.00%
|
100.00%
|
| Primer 416 | Thermotoga | AGGTCGCAACGTGGTGAT | GTCACGGCAGATGTAGCAGA | 59.26 | 59.83 | 165 | 114 |
100.00%
|
60.00%
|
| Primer 417 | Thermotoga | AGGTCGCAACGTGGTGAT | TTTCCTCAGTCACGGCAGA | 59.26 | 58.56 | 173 | 114 |
100.00%
|
80.00%
|
| Primer 418 | Thermotoga | TTCACGTTTGCTGTGCGC | TCTTGCATCGTTCGCTGC | 59.98 | 58.83 | 249 | 114 |
100.00%
|
40.00%
|
| Primer 419 | Thermotoga | TTCACGTTTGCTGTGCGC | TCTCCTCGTCGCTTCTTGC | 59.98 | 59.79 | 299 | 114 |
100.00%
|
40.00%
|
| Primer 420 | Thermotoga | TTCACGTTTGCTGTGCGC | TTCTTGCATCGTTCGCTGC | 59.98 | 59.50 | 250 | 114 |
100.00%
|
40.00%
|