| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 431 | Thermotoga | CGTACGAAGCCACGAGACA | AAGAAACACCCGTCGCCA | 59.79 | 59.49 | 297 | 113 |
100.00%
|
80.00%
|
| Primer 432 | Thermotoga | CGACAGCGACAAAGAAGCG | TGGCCATGTTGTGAGCCA | 59.87 | 59.48 | 293 | 113 |
100.00%
|
80.00%
|
| Primer 433 | Thermotoga | AGCCACCGAACATAGCCA | AAACCGCTTCCTGCCCAT | 58.60 | 59.56 | 288 | 113 |
100.00%
|
40.00%
|
| Primer 434 | Thermotoga | TGGCGAAGTACCAGGTCAC | ATTCGCTCGGACCCACTTC | 59.33 | 59.78 | 213 | 113 |
100.00%
|
100.00%
|
| Primer 435 | Thermotoga | GACCACCAAGGCCATTCCA | ACCTCTGTGGCAGGTGTTG | 59.92 | 59.85 | 210 | 113 |
100.00%
|
40.00%
|
| Primer 436 | Thermotoga | ACCACCAAGGCCATTCCA | ACCTCTGTGGCAGGTGTTG | 58.73 | 59.85 | 209 | 113 |
100.00%
|
40.00%
|
| Primer 437 | Thermotoga | ACGGCAAAACGGTTGCAC | AGAACGCACTCTGCCCTTC | 59.90 | 60.00 | 177 | 113 |
100.00%
|
100.00%
|
| Primer 438 | Thermotoga | TGCTCAAGTTGGAGGCCA | TCGGGTGTTTTCTGCGTCG | 58.75 | 60.95 | 181 | 113 |
100.00%
|
80.00%
|
| Primer 439 | Thermotoga | TGCTCAAGTTGGAGGCCA | TCCCGTGAACTTCCAGTCC | 58.75 | 58.94 | 273 | 113 |
100.00%
|
80.00%
|
| Primer 440 | Thermotoga | TGCTCAAGTTGGAGGCCA | CATCGGGTGTTTTCTGCGTC | 58.75 | 59.83 | 183 | 113 |
100.00%
|
80.00%
|