| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 461 | Thermotoga | TCAAGAGCGGAGGGTTCAC | TCACCGTGAAAGCACCGT | 59.33 | 59.50 | 204 | 112 |
100.00%
|
60.00%
|
| Primer 462 | Thermotoga | AGGGTGCAGCTTCTCGATG | AACATGCACCGCACCCAT | 59.78 | 60.28 | 226 | 112 |
100.00%
|
40.00%
|
| Primer 463 | Thermotoga | TCGTACACGACTCAGCACG | TGCCCATTCGCCACCATT | 59.79 | 59.96 | 236 | 112 |
100.00%
|
80.00%
|
| Primer 464 | Thermotoga | AGCCAGAGTGTGAGAAGCG | TGCCCATTCGCCACCATT | 59.71 | 59.96 | 294 | 112 |
100.00%
|
80.00%
|
| Primer 465 | Thermotoga | TCTTCTCCACCCGAGGGATT | ACGTGCTTCGCGAGAACA | 59.96 | 59.97 | 239 | 112 |
100.00%
|
80.00%
|
| Primer 466 | Thermotoga | AACGTCGAATGGGTGCGT | AGCGCATCCATGATCTCGT | 59.97 | 59.26 | 295 | 112 |
100.00%
|
80.00%
|
| Primer 467 | Thermotoga | AAAGTGCAAGCCCTGTGC | TCGCTCATACTGCCACACC | 58.87 | 59.78 | 151 | 112 |
100.00%
|
60.00%
|
| Primer 468 | Thermotoga | ACATCGTGGCTCTGGCAA | TCGCCACAAGTCTTGCGT | 59.25 | 59.89 | 222 | 112 |
100.00%
|
80.00%
|
| Primer 469 | Thermotoga | AACATCGTGGCTCTGGCA | TCGCCACAAGTCTTGCGT | 59.25 | 59.89 | 223 | 112 |
100.00%
|
80.00%
|
| Primer 470 | Thermotoga | ACGGATGAGGGAGGAAGCT | TCGCCACAAGTCTTGCGT | 60.00 | 59.89 | 295 | 112 |
100.00%
|
60.00%
|