| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 481 | Thermotoga | AGTGTGCGGAAAAGCTCCT | ACACCTCCGAAACTTGTCCAA | 59.55 | 59.79 | 194 | 111 |
100.00%
|
80.00%
|
| Primer 482 | Thermotoga | AGTGTGCGGAAAAGCTCCT | CACACCTCCGAAACTTGTCC | 59.55 | 58.77 | 195 | 111 |
100.00%
|
80.00%
|
| Primer 483 | Thermotoga | TTCTTCGCGTGGCTCGTT | TGATTGCTGCGTTGGTGG | 59.97 | 58.65 | 256 | 111 |
100.00%
|
80.00%
|
| Primer 484 | Thermotoga | TTCTTCGCGTGGCTCGTT | CCCCAGAAACCAGCCATCA | 59.97 | 59.62 | 188 | 111 |
100.00%
|
80.00%
|
| Primer 485 | Thermotoga | TTCTTCGCGTGGCTCGTT | ACACTCCCCAGAAACCAGC | 59.97 | 59.54 | 193 | 111 |
100.00%
|
80.00%
|
| Primer 486 | Thermotoga | TTCTTCGCGTGGCTCGTT | TGGTGGGCTCTGTGAACA | 59.97 | 58.35 | 244 | 111 |
100.00%
|
80.00%
|
| Primer 487 | Thermotoga | TTCTTCGCGTGGCTCGTT | TCCCCAGAAACCAGCCATC | 59.97 | 59.31 | 189 | 111 |
100.00%
|
80.00%
|
| Primer 488 | Thermotoga | TCGCACACGTTCCCATGT | TCACGTATTCGGCACCGTT | 59.58 | 59.71 | 234 | 111 |
100.00%
|
100.00%
|
| Primer 489 | Thermotoga | CTCAGTGCACAACCCACCT | AGCATCGTTTCCGCACCA | 59.85 | 59.97 | 183 | 111 |
100.00%
|
40.00%
|
| Primer 490 | Thermotoga | TCAGTGCACAACCCACCT | AGCATCGTTTCCGCACCA | 58.67 | 59.97 | 182 | 111 |
100.00%
|
40.00%
|