| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Thermotoga | TCAGTGCACAACCCACCT | AGCATCGTTTCCGCACCA | 58.67 | 59.97 | 182 | 111 |
100.00%
|
40.00%
|
| Primer 502 | Thermotoga | AGTGTGCGGAAAAGCTCCT | ACACCTCCGAAACTTGTCCA | 59.55 | 59.17 | 194 | 111 |
100.00%
|
80.00%
|
| Primer 503 | Thermotoga | AGTGTGCGGAAAAGCTCCT | CCTCCGAAACTTGTCCAACG | 59.55 | 59.13 | 191 | 111 |
100.00%
|
80.00%
|
| Primer 504 | Thermotoga | AGTGTGCGGAAAAGCTCCT | TCACACCTCCGAAACTTGTCC | 59.55 | 60.20 | 196 | 111 |
100.00%
|
80.00%
|
| Primer 505 | Thermotoga | AGTGTGCGGAAAAGCTCCT | ACACCTCCGAAACTTGTCCAA | 59.55 | 59.79 | 194 | 111 |
100.00%
|
80.00%
|
| Primer 506 | Thermotoga | AGTGTGCGGAAAAGCTCCT | CACACCTCCGAAACTTGTCC | 59.55 | 58.77 | 195 | 111 |
100.00%
|
80.00%
|
| Primer 507 | Thermotoga | GCTCACAACCAGAGGGCTT | TACACAGGCGAACGCGTT | 59.93 | 59.97 | 291 | 111 |
100.00%
|
80.00%
|
| Primer 508 | Thermotoga | ACGGTGCTCACAACCAGA | TACACAGGCGAACGCGTT | 58.77 | 59.97 | 296 | 111 |
100.00%
|
80.00%
|
| Primer 509 | Thermotoga | CTGGCGTGTGATGAGACCT | TTCGCACAGAGTCCGCAT | 59.41 | 59.34 | 153 | 111 |
100.00%
|
80.00%
|
| Primer 510 | Thermotoga | TTGTTCGCTCAACTGCCCT | AAACGACGTCAGCCAGCT | 59.85 | 59.58 | 254 | 111 |
100.00%
|
80.00%
|