| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Thermotoga | ATCCCGACCATTGGTGTGG | ATGTGTGCCAGTCTGGTGG | 59.70 | 59.93 | 251 | 111 |
100.00%
|
80.00%
|
| Primer 512 | Thermotoga | GCAAATGTGAGCACACCCG | CCCTCAGCACGGCATTGTA | 60.08 | 60.08 | 292 | 111 |
100.00%
|
80.00%
|
| Primer 513 | Thermotoga | TGGTGATGACCGCAAGTCC | CGTTCCCCTCAACAAGCCT | 60.00 | 59.93 | 246 | 111 |
100.00%
|
80.00%
|
| Primer 514 | Thermotoga | ACTGGGTTGGCGTTGTGA | CACAACCTCGTCGAGCTGA | 59.41 | 59.71 | 212 | 110 |
100.00%
|
100.00%
|
| Primer 515 | Thermotoga | ACTGGGTTGGCGTTGTGA | TCACAACCTCGTCGAGCTG | 59.41 | 59.71 | 213 | 110 |
100.00%
|
100.00%
|
| Primer 516 | Thermotoga | CGCGAGATCCAGGAACTCA | GGTGCTCTGTAACCTGACCC | 59.19 | 60.04 | 226 | 110 |
100.00%
|
40.00%
|
| Primer 517 | Thermotoga | ACTCGGCTACACCACAACG | ACGGATCGTTCACGCTGA | 60.01 | 59.04 | 182 | 110 |
100.00%
|
80.00%
|
| Primer 518 | Thermotoga | AGGCCACCTACCCTGTGAT | ACGGATCGTTCACGCTGA | 59.92 | 59.04 | 237 | 110 |
100.00%
|
40.00%
|
| Primer 519 | Thermotoga | AAGGCCACCTACCCTGTGA | ACGGATCGTTCACGCTGA | 60.15 | 59.04 | 238 | 110 |
100.00%
|
40.00%
|
| Primer 520 | Thermotoga | AGCCTGGCTTGATGGAAGT | AAGGAATGTCCGCTCCTGT | 58.92 | 58.62 | 228 | 110 |
100.00%
|
40.00%
|