| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Thermotoga | TACTGCGCAAGAGAGGCAC | TCCTCGCAACCGCAAAGT | 60.08 | 59.89 | 193 | 110 |
100.00%
|
100.00%
|
| Primer 522 | Thermotoga | ATGCGAACGGTCAGCTCA | CCGCTTTTCGCATCGCAT | 59.34 | 59.60 | 267 | 110 |
100.00%
|
80.00%
|
| Primer 523 | Thermotoga | CGCGAGATCCAGGAACTCA | GGTGCTCTGTAACCTGACCC | 59.19 | 60.04 | 226 | 110 |
100.00%
|
40.00%
|
| Primer 524 | Thermotoga | TCAAGATCGGCTTCTGGGC | AGGATCGCAGCAAGGAAGG | 59.78 | 59.78 | 220 | 110 |
100.00%
|
100.00%
|
| Primer 525 | Thermotoga | GTGAAGTGTGCGGAAAGGC | ACACCTCCGAAACCTGACC | 59.72 | 59.25 | 198 | 110 |
100.00%
|
100.00%
|
| Primer 526 | Thermotoga | AGCCTGGCTTGATGGAAGT | AAGGAATGTCCGCTCCTGT | 58.92 | 58.62 | 228 | 110 |
100.00%
|
40.00%
|
| Primer 527 | Thermotoga | ACTCCCGTCACCACCTTCA | TTTGCGACAACCTCCGGA | 60.46 | 59.18 | 230 | 110 |
100.00%
|
60.00%
|
| Primer 528 | Thermotoga | TCAAGATCGGCTTCTGGGC | AGGATCGCAGCAAGGAAGG | 59.78 | 59.78 | 220 | 110 |
100.00%
|
100.00%
|
| Primer 529 | Thermotoga | TGGTGTTGCTCGGCATGT | AGTCCGTCCGTCAGGTTCA | 59.89 | 60.53 | 265 | 110 |
100.00%
|
60.00%
|
| Primer 530 | Thermotoga | ATGCGAACGGTCAGCTCA | CCGCTTTTCGCATCGCAT | 59.34 | 59.60 | 267 | 110 |
100.00%
|
80.00%
|