| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Thermotoga | CTCGCCTACAACGTGGTCA | TCACCAGCAGGCACGTTT | 59.71 | 59.81 | 278 | 109 |
100.00%
|
60.00%
|
| Primer 542 | Thermotoga | ACTCGCCTACAACGTGGTC | TCACCAGCAGGCACGTTT | 59.71 | 59.81 | 279 | 109 |
100.00%
|
60.00%
|
| Primer 543 | Thermotoga | TCGCCTACAACGTGGTCA | TCACCAGCAGGCACGTTT | 58.55 | 59.81 | 277 | 109 |
100.00%
|
60.00%
|
| Primer 544 | Thermotoga | ACTCGCCTACAACGTGGT | TCACCAGCAGGCACGTTT | 58.55 | 59.81 | 279 | 109 |
100.00%
|
60.00%
|
| Primer 545 | Thermotoga | AACTCGCCTACAACGTGGT | TCACCAGCAGGCACGTTT | 59.26 | 59.81 | 280 | 109 |
100.00%
|
60.00%
|
| Primer 546 | Thermotoga | TCAAAGGGCACGATGCGA | ACGATCTTTCCACCGTCCG | 59.65 | 59.79 | 172 | 109 |
100.00%
|
100.00%
|
| Primer 547 | Thermotoga | TCAAAGGGCACGATGCGA | GACCCTGATTCGGTCGGTT | 59.65 | 59.40 | 296 | 109 |
100.00%
|
80.00%
|
| Primer 548 | Thermotoga | AGTGCGGAAGGAAGGGTTG | TGCTGCTTCTTTGCGTGC | 59.93 | 59.67 | 203 | 109 |
100.00%
|
60.00%
|
| Primer 549 | Thermotoga | GCACTTTCTTCTCCACCCGA | ACGTGCTTCGCGAGAACA | 59.97 | 59.97 | 245 | 109 |
100.00%
|
80.00%
|
| Primer 550 | Thermotoga | TGGCTCTGGCAAAAGACGA | TCGCCACAAGTCTTGCGT | 59.55 | 59.89 | 216 | 109 |
100.00%
|
80.00%
|