| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 561 | Thermotoga | AGAACGACACGCGAACGA | AGTCCCCTGAAGTCCCTCA | 59.67 | 59.13 | 201 | 109 |
100.00%
|
60.00%
|
| Primer 562 | Thermotoga | AGAACGACACGCGAACGA | CTTGGGCAGCACGATGTTT | 59.67 | 59.04 | 181 | 109 |
100.00%
|
60.00%
|
| Primer 563 | Thermotoga | AGAACGACACGCGAACGA | CCTTGGGCAGCACGATGTT | 59.67 | 60.97 | 182 | 109 |
100.00%
|
60.00%
|
| Primer 564 | Thermotoga | AGAACGACACGCGAACGA | ATCCGGACTCAGTTCAGGGA | 59.67 | 59.96 | 283 | 109 |
100.00%
|
60.00%
|
| Primer 565 | Thermotoga | AGAACGACACGCGAACGA | CATCCGGACTCAGTTCAGGG | 59.67 | 59.82 | 284 | 109 |
100.00%
|
60.00%
|
| Primer 566 | Thermotoga | ACGACACACCGTTCACGA | ACCGAAGACGTTTCCGCT | 59.20 | 59.27 | 182 | 109 |
100.00%
|
80.00%
|
| Primer 567 | Thermotoga | TGTTTTGCCCAGGTGCCA | TCCTCAAGCCAGCAAACGA | 60.04 | 59.55 | 251 | 109 |
100.00%
|
40.00%
|
| Primer 568 | Thermotoga | ATCACGCCTCCTGCAGAAC | TCGAGCGATCTTACACCGAT | 60.08 | 58.69 | 198 | 108 |
100.00%
|
80.00%
|
| Primer 569 | Thermotoga | GTGAGCGATCGACCTGTGT | AGCACCGTTGACCGCTAT | 59.79 | 59.02 | 239 | 108 |
100.00%
|
80.00%
|
| Primer 570 | Thermotoga | ACCACCTTCACCATAGCGG | TTTGCGACAACCTCCGGA | 59.40 | 59.18 | 221 | 108 |
100.00%
|
60.00%
|