| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Thermotoga | GCAAATGTGAGCACACCCG | CCCTCAGCACGGCATTGTA | 60.08 | 60.08 | 292 | 111 |
100.00%
|
80.00%
|
| Primer 492 | Thermotoga | ATCCCGACCATTGGTGTGG | ATGTGTGCCAGTCTGGTGG | 59.70 | 59.93 | 251 | 111 |
100.00%
|
80.00%
|
| Primer 493 | Thermotoga | TGTTCGCTCAACTGCCCT | AAACGACGTCAGCCAGCT | 59.17 | 59.58 | 253 | 111 |
100.00%
|
80.00%
|
| Primer 494 | Thermotoga | TGCTGCCTTTGACGCACT | AAGGCCTGACCGGTTCCTA | 60.20 | 60.23 | 186 | 111 |
100.00%
|
60.00%
|
| Primer 495 | Thermotoga | AGCGGTTGTGGAGCAGTT | GCGAGCTCGAGGAAACCTT | 59.49 | 60.08 | 153 | 111 |
100.00%
|
100.00%
|
| Primer 496 | Thermotoga | AGCGGTTGTGGAGCAGTT | TGTTCTTGCGAGCTCGAGG | 59.49 | 60.08 | 160 | 111 |
100.00%
|
100.00%
|
| Primer 497 | Thermotoga | AGCGGTTGTGGAGCAGTT | GTTCTTGCGAGCTCGAGGA | 59.49 | 59.79 | 159 | 111 |
100.00%
|
100.00%
|
| Primer 498 | Thermotoga | AGCGGTTGTGGAGCAGTT | TTGCGAGCTCGAGGAAACC | 59.49 | 60.37 | 155 | 111 |
100.00%
|
100.00%
|
| Primer 499 | Thermotoga | ACCACCTGCGGAAACAGTT | AGCCGTTCTGCTGCATCT | 59.77 | 59.33 | 211 | 111 |
100.00%
|
60.00%
|
| Primer 500 | Thermotoga | CTCAGTGCACAACCCACCT | AGCATCGTTTCCGCACCA | 59.85 | 59.97 | 183 | 111 |
100.00%
|
40.00%
|