| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 671 | Thermotoga | TGGCGAGCAGAGAGGAGAT | TGTAGCTGACGATGTCGCC | 60.08 | 59.86 | 225 | 105 |
100.00%
|
100.00%
|
| Primer 672 | Thermotoga | CCCAAAGGATGCCGAACAC | TCGCTGCGTTCGCTTCAT | 59.12 | 60.43 | 161 | 105 |
100.00%
|
60.00%
|
| Primer 673 | Thermotoga | CACCCAAAGGATGCCGAAC | TCGCTGCGTTCGCTTCAT | 59.12 | 60.43 | 163 | 105 |
100.00%
|
60.00%
|
| Primer 674 | Thermotoga | AACGGCAGGCGTTCACAT | GATGCCTTCGTCCGTCACT | 60.28 | 59.78 | 178 | 105 |
100.00%
|
60.00%
|
| Primer 675 | Thermotoga | AACGGCAGGCGTTCACAT | ATGCCTTCGTCCGTCACT | 60.28 | 58.62 | 177 | 105 |
100.00%
|
60.00%
|
| Primer 676 | Thermotoga | ACCGGTGGGATTCCCTACA | TTGCGTGGTTGGGGTTCT | 59.92 | 59.40 | 188 | 105 |
100.00%
|
60.00%
|
| Primer 677 | Thermotoga | TCAGGCGATGGAAAGCACT | TCCACCCTGATCACGGGAT | 59.32 | 60.00 | 294 | 105 |
100.00%
|
80.00%
|
| Primer 678 | Thermotoga | ACAACTGCCAGCGCTCAT | CTGGTTGTTGTGGCCACAG | 59.97 | 59.26 | 242 | 105 |
100.00%
|
80.00%
|
| Primer 679 | Thermotoga | ACAACTGCCAGCGCTCAT | GTGTCTTCCTTGTCGCTGC | 59.97 | 59.14 | 295 | 105 |
100.00%
|
60.00%
|
| Primer 680 | Thermotoga | TGAGAAAGCCGTGGACACT | TCCCAATGGAGCAATCGGTC | 58.87 | 60.11 | 166 | 105 |
100.00%
|
60.00%
|