| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 681 | Thermotoga | TGGTGATGACCGCAAGTCC | ACGTTCCCCTCAACAAGCC | 60.00 | 60.23 | 247 | 105 |
100.00%
|
80.00%
|
| Primer 682 | Thermotoga | AGATCCTGGGAGCGGACAT | AGTCACGTGAGCTGGCTTC | 60.07 | 60.01 | 164 | 105 |
100.00%
|
80.00%
|
| Primer 683 | Thermotoga | ATTGCATCGGACCACGCA | TCGCGAGTCTTGCCATGT | 60.05 | 59.34 | 274 | 105 |
100.00%
|
80.00%
|
| Primer 684 | Thermotoga | TCGACAACGGTCAGCACA | CCACTTCCGCTACGTTGGA | 59.19 | 59.71 | 165 | 104 |
100.00%
|
80.00%
|
| Primer 685 | Thermotoga | TCGACAACGGTCAGCACA | ATCGGGATCCTGATGCGTG | 59.19 | 59.63 | 215 | 104 |
100.00%
|
80.00%
|
| Primer 686 | Thermotoga | TCTTGCCCTCTGCGGAAA | TCCAAGATGGCAGCTGCA | 58.84 | 59.24 | 255 | 104 |
100.00%
|
40.00%
|
| Primer 687 | Thermotoga | CGATCATTGTGGGAGTGGCA | TCACTGCTGCTGGGCAAA | 60.39 | 59.80 | 247 | 104 |
100.00%
|
60.00%
|
| Primer 688 | Thermotoga | ACGATCATTGTGGGAGTGGC | TCACTGCTGCTGGGCAAA | 60.39 | 59.80 | 248 | 104 |
100.00%
|
60.00%
|
| Primer 689 | Thermotoga | TGCCCACTTACACGTTCAGG | ACAGCTTCCACCACACGAG | 60.25 | 59.93 | 182 | 104 |
100.00%
|
80.00%
|
| Primer 690 | Thermotoga | GCGGTCTCATCGACAGACA | TCGTTCCTGAAGCCGTGT | 59.49 | 58.87 | 154 | 104 |
100.00%
|
80.00%
|