| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 701 | Thermotoga | ACGCGAACGAAGAGGCTT | ATCTGTTTCCCGTGTGGCA | 59.66 | 59.55 | 207 | 103 |
100.00%
|
80.00%
|
| Primer 702 | Thermotoga | ACGCGAACGAAGAGGCTT | TCTGTTTCCCGTGTGGCAC | 59.66 | 60.53 | 206 | 103 |
100.00%
|
80.00%
|
| Primer 703 | Thermotoga | AGACAAAGCCACGCAGGT | TCTTCCCACGGCTCGTAGA | 59.49 | 60.00 | 234 | 103 |
100.00%
|
60.00%
|
| Primer 704 | Thermotoga | ACCAGGCGTTCGACATCA | TGGTTTGCCTGGCCACTT | 58.94 | 59.72 | 225 | 103 |
100.00%
|
40.00%
|
| Primer 705 | Thermotoga | ACCAGGCGTTCGACATCAG | TGGTTTGCCTGGCCACTT | 60.08 | 59.72 | 225 | 103 |
100.00%
|
40.00%
|
| Primer 706 | Thermotoga | AACGTCCGTGTGCTTGGA | CAGCCTTCTGTCTCCGCTT | 59.50 | 59.71 | 279 | 103 |
100.00%
|
40.00%
|
| Primer 707 | Thermotoga | GAGAAAGCGCTGGGGAAGA | AAGGCCACCACGTTTGCT | 59.70 | 60.12 | 288 | 103 |
100.00%
|
40.00%
|
| Primer 708 | Thermotoga | GCCATGCTCGAATCGGAGA | TTCCGGAGCAACGTAGAGC | 59.93 | 59.79 | 252 | 103 |
100.00%
|
80.00%
|
| Primer 709 | Thermotoga | GCGGATGAAGCAGAGCAGA | ACTGTGCGACGGTGTTGT | 60.15 | 59.81 | 205 | 103 |
100.00%
|
80.00%
|
| Primer 710 | Thermotoga | AACGGCAGGCGTTCACAT | TGCCTTCGTCCGTCACTT | 60.28 | 58.87 | 176 | 103 |
100.00%
|
60.00%
|