| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 721 | Thermotoga | CTGCTGGCCTCGATGTGAT | GCCAATTCACGGAGAGCCA | 59.85 | 60.38 | 292 | 102 |
100.00%
|
40.00%
|
| Primer 722 | Thermotoga | TTTCCGGTGAACGTGGTGG | TCGTTCATGTGACGCACCA | 60.52 | 59.93 | 215 | 102 |
100.00%
|
60.00%
|
| Primer 723 | Thermotoga | AGGAAATTAGCGCTGGAAGGA | ACTGAGGCATACCGTTTCCG | 59.44 | 60.11 | 154 | 102 |
100.00%
|
40.00%
|
| Primer 724 | Thermotoga | AGGAAATTAGCGCTGGAAGGA | CTGAGGCATACCGTTTCCG | 59.44 | 58.32 | 153 | 102 |
100.00%
|
40.00%
|
| Primer 725 | Thermotoga | TCGCTCAATCACCGCTGA | TCCCAGCCTCTTTCTTGCG | 59.03 | 60.00 | 178 | 102 |
100.00%
|
40.00%
|
| Primer 726 | Thermotoga | ACAACGGTGCGATGGTGT | AAGGTTCGGTTCAACGCG | 59.89 | 58.67 | 215 | 102 |
100.00%
|
40.00%
|
| Primer 727 | Thermotoga | TTGCGCTCATGCTCTGGT | CAGAGAGGCGTTGTTTGCG | 59.65 | 59.79 | 206 | 102 |
100.00%
|
100.00%
|
| Primer 728 | Thermotoga | CAGCGACAAAGAAGCGGTG | TGGCCATGTTGTGAGCCA | 59.79 | 59.48 | 290 | 102 |
100.00%
|
80.00%
|
| Primer 729 | Thermotoga | TGCCGTTGAGTGCGAATG | TGGCAAACGAAGGTTCCCA | 58.75 | 59.77 | 243 | 102 |
100.00%
|
80.00%
|
| Primer 730 | Thermotoga | AACAGGGAAGCCGTTTTCG | GGCCACCCACACTGAAAGA | 58.67 | 59.85 | 157 | 102 |
100.00%
|
80.00%
|