| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 711 | Thermotoga | TGGGAATCTGTGCCTACGC | AACAACCATCGGCAGCCA | 59.78 | 59.88 | 212 | 103 |
100.00%
|
80.00%
|
| Primer 712 | Thermotoga | ACAACTGCCAGCGCTCAT | TGGTTGTTGTGGCCACAGA | 59.97 | 59.70 | 241 | 103 |
100.00%
|
80.00%
|
| Primer 713 | Thermotoga | AGGCAGTACAGGGTCGAAGA | TGGCTTTGGCGTGTTCCA | 60.25 | 60.12 | 184 | 103 |
100.00%
|
60.00%
|
| Primer 714 | Thermotoga | GGCAGTACAGGGTCGAAGA | TGGCTTTGGCGTGTTCCA | 58.73 | 60.12 | 183 | 103 |
100.00%
|
60.00%
|
| Primer 715 | Thermotoga | TACGACCGGTGCTCTCCTT | TGGCGACGGGAATGTTGT | 60.30 | 59.57 | 215 | 103 |
100.00%
|
80.00%
|
| Primer 716 | Thermotoga | ACGATCGAGCCACAACAGA | CCGCTTCTTCGGGATGAGA | 59.04 | 59.18 | 240 | 103 |
100.00%
|
80.00%
|
| Primer 717 | Thermotoga | TCGAGCCACAACAGACGAAA | CCGCTTCTTCGGGATGAGA | 59.90 | 59.18 | 236 | 103 |
100.00%
|
80.00%
|
| Primer 718 | Thermotoga | CCGGAGTAGCCAAGGGATTG | CCGCTTCTTCGGGATGAGA | 60.18 | 59.18 | 204 | 103 |
100.00%
|
60.00%
|
| Primer 719 | Thermotoga | CCGGATTCGAACATCTGCG | GCGCTTCCTGATTTTCGGC | 59.07 | 60.23 | 192 | 102 |
100.00%
|
80.00%
|
| Primer 720 | Thermotoga | TGCCGTTGAGTGCGAATG | TGGCAAACGAAGGTTCCCA | 58.75 | 59.77 | 243 | 102 |
100.00%
|
80.00%
|