| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 741 | Thermotoga | TCGACGACGTGGTGAGAGT | AAAGCACCTGACTCGCGT | 60.60 | 59.58 | 159 | 101 |
100.00%
|
80.00%
|
| Primer 742 | Thermotoga | GGGGACATTCATGGGTGCT | CTCCGAAGCTTTCCACCGT | 59.69 | 60.00 | 274 | 101 |
100.00%
|
80.00%
|
| Primer 743 | Thermotoga | GGGGACATTCATGGGTGCT | TCCGAAGCTTTCCACCGT | 59.69 | 58.86 | 273 | 101 |
100.00%
|
80.00%
|
| Primer 744 | Thermotoga | ACGAACAGAGCACCAGCAG | AAGGCAAAGCGCAGATGC | 60.30 | 59.43 | 189 | 101 |
100.00%
|
80.00%
|
| Primer 745 | Thermotoga | TTGGGAAAGGCGTGTCGT | AAGAACCTTCGCAGCCGT | 59.49 | 59.57 | 176 | 101 |
100.00%
|
80.00%
|
| Primer 746 | Thermotoga | CGAGACCACCAAGGCCATT | ACCTCTGTGGCAGGTGTTG | 60.00 | 59.85 | 213 | 101 |
100.00%
|
40.00%
|
| Primer 747 | Thermotoga | TCAGAGTGTACGCGTCCAG | TCACCGTGAAAGCACCGT | 59.13 | 59.50 | 159 | 101 |
100.00%
|
40.00%
|
| Primer 748 | Thermotoga | CTCAGAGTGTACGCGTCCA | TCACCGTGAAAGCACCGT | 59.13 | 59.50 | 160 | 101 |
100.00%
|
40.00%
|
| Primer 749 | Thermotoga | ACTCAGAGTGTACGCGTCC | TCACCGTGAAAGCACCGT | 59.13 | 59.50 | 161 | 101 |
100.00%
|
40.00%
|
| Primer 750 | Thermotoga | CTCAAGAGCGGAGGGTTCA | TCACCGTGAAAGCACCGT | 59.02 | 59.50 | 205 | 101 |
100.00%
|
60.00%
|