| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 751 | Thermotoga | CTCAGAGTGTACGCGTCCAG | TCACCGTGAAAGCACCGT | 60.18 | 59.50 | 160 | 101 |
100.00%
|
40.00%
|
| Primer 752 | Thermotoga | AACTCAGAGTGTACGCGTCC | TCACCGTGAAAGCACCGT | 59.76 | 59.50 | 162 | 101 |
100.00%
|
40.00%
|
| Primer 753 | Thermotoga | TCGTGGGTGTGAGCGAAA | AGAACTTCAGGCTCGCGT | 59.18 | 58.95 | 288 | 101 |
100.00%
|
80.00%
|
| Primer 754 | Thermotoga | ACAGGATCCGTTGAGGTGC | TCCGGTTTCTTGTGTCTCAGG | 59.70 | 59.93 | 164 | 101 |
100.00%
|
80.00%
|
| Primer 755 | Thermotoga | TCGGTGTTTCTCTGCCACC | AGGTGGAAAGCGCTTCGT | 59.93 | 59.57 | 234 | 101 |
100.00%
|
60.00%
|
| Primer 756 | Thermotoga | GCAGAAGAGGCGAGCAATG | TCCTCGGGTCCAGATGTGT | 59.28 | 59.92 | 226 | 101 |
100.00%
|
40.00%
|
| Primer 757 | Thermotoga | TTGCCCAGCTCAGCGAAT | TGCAGCGATGATCTCCGT | 59.65 | 58.79 | 272 | 101 |
100.00%
|
40.00%
|
| Primer 758 | Thermotoga | TGGTGTTCCACGCACAGA | ACCCCACTTGTGAAGCGT | 59.10 | 59.08 | 258 | 101 |
100.00%
|
60.00%
|
| Primer 759 | Thermotoga | AGTCATCGGAGAAGTGGCG | AAAGCACCTGACTCGCGT | 59.49 | 59.58 | 248 | 101 |
100.00%
|
80.00%
|
| Primer 760 | Thermotoga | TTCGACGACGTGGTGAGAG | AAAGCACCTGACTCGCGT | 59.43 | 59.58 | 160 | 101 |
100.00%
|
80.00%
|