| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 941 | Thermotoga | TATGCCGTACCACACAGGC | TTCACACCGAGCACTGCA | 59.78 | 59.50 | 189 | 94 |
100.00%
|
100.00%
|
| Primer 942 | Thermotoga | ACTTTGCGATCGCGTTGC | GCCGTGCCGTTTTCAAACT | 59.83 | 59.93 | 168 | 94 |
100.00%
|
40.00%
|
| Primer 943 | Thermotoga | TTCGAAGCGCTTCTGGCT | GGCCTCCTTTTCCGCTTCT | 59.66 | 60.00 | 297 | 94 |
100.00%
|
40.00%
|
| Primer 944 | Thermotoga | TTGTTGTCGCAGCGCTTC | ATTGCCTCAAGGAGCGCA | 59.37 | 59.65 | 286 | 94 |
100.00%
|
80.00%
|
| Primer 945 | Thermotoga | AGTGGTTGTTGCCGTGGA | CTTGCCCTCGACTGCTTCT | 59.41 | 59.71 | 253 | 94 |
100.00%
|
80.00%
|
| Primer 946 | Thermotoga | AGTGGTTGTTGCCGTGGA | ATTCAACCCCTCCCTTGCC | 59.41 | 59.61 | 266 | 94 |
100.00%
|
80.00%
|
| Primer 947 | Thermotoga | TCGTGAGATTGTCGCTGGAG | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 172 | 94 |
100.00%
|
80.00%
|
| Primer 948 | Thermotoga | CTCGTGAGATTGTCGCTGGA | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 173 | 94 |
100.00%
|
80.00%
|
| Primer 949 | Thermotoga | TCTCGTGAGATTGTCGCTGG | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 174 | 94 |
100.00%
|
80.00%
|
| Primer 950 | Thermotoga | TCGTGAGATTGTCGCTGGA | TCGTCTGGCGACTTTGCA | 58.74 | 59.58 | 172 | 94 |
100.00%
|
80.00%
|