| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 961 | Thermotoga | GCTCGTACTGACAGTGGCA | AGACCGTAGCCGAAACCTC | 59.71 | 59.11 | 195 | 94 |
100.00%
|
80.00%
|
| Primer 962 | Thermotoga | TTCAGGCGATGGAAAGCACT | TCCACCCTGATCACGGGAT | 59.96 | 60.00 | 295 | 94 |
100.00%
|
80.00%
|
| Primer 963 | Thermotoga | AACTGGAGTGCGGAAGGTG | TCTGAGAAAGTGGCTCGCC | 59.93 | 59.71 | 261 | 94 |
100.00%
|
100.00%
|
| Primer 964 | Thermotoga | TTCACAACGGACACGGCA | TGGAACAACGGTGCTTCCC | 59.81 | 60.53 | 297 | 94 |
100.00%
|
40.00%
|
| Primer 965 | Thermotoga | TCGTGAGATTGTCGCTGGAG | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 172 | 94 |
100.00%
|
80.00%
|
| Primer 966 | Thermotoga | CTCGTGAGATTGTCGCTGGA | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 173 | 94 |
100.00%
|
80.00%
|
| Primer 967 | Thermotoga | TCTCGTGAGATTGTCGCTGG | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 174 | 94 |
100.00%
|
80.00%
|
| Primer 968 | Thermotoga | TCGTGAGATTGTCGCTGGA | TCGTCTGGCGACTTTGCA | 58.74 | 59.58 | 172 | 94 |
100.00%
|
80.00%
|
| Primer 969 | Thermotoga | CGTGAGATTGTCGCTGGAG | TCGTCTGGCGACTTTGCA | 58.34 | 59.58 | 171 | 94 |
100.00%
|
80.00%
|
| Primer 970 | Thermotoga | TGAGAAAGCCGTGGACACT | CCCAATGGAGCAATCGGTC | 58.87 | 58.59 | 165 | 94 |
100.00%
|
60.00%
|