| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Alkalihalobacillus | AACGCGTTTCCAAGCAGC | TCGCTGTGAACGTGGTGT | 59.67 | 59.50 | 184 | 121 |
100.00%
|
50.00%
|
| Primer 22 | Alkalihalobacillus | ATGGGAGCCGATCATGCTG | AACCTGCCCAGTTCCTTGG | 59.93 | 59.85 | 156 | 121 |
100.00%
|
50.00%
|
| Primer 23 | Alkalihalobacillus | TGAGCCCAAGTCTCGTAGC | TCCGATTGGTAAGGCGTTAGG | 59.11 | 59.86 | 274 | 121 |
100.00%
|
50.00%
|
| Primer 24 | Alkalihalobacillus | ACGCTTGTCGACGATGCA | TTCGCCAATCTCCCGTAGC | 60.05 | 59.86 | 297 | 120 |
100.00%
|
50.00%
|
| Primer 25 | Alkalihalobacillus | ACGCTTGTCGACGATGCA | CAACGGCTACGACATTGGC | 60.05 | 59.58 | 262 | 120 |
100.00%
|
50.00%
|
| Primer 26 | Alkalihalobacillus | ACGCTTGTCGACGATGCA | ACGGCTACGACATTGGCTG | 60.05 | 60.45 | 260 | 120 |
100.00%
|
50.00%
|
| Primer 27 | Alkalihalobacillus | ACGGAGTTGTCGCAGATGG | ACCACAACGCCATCTGCA | 60.08 | 59.89 | 196 | 120 |
100.00%
|
50.00%
|
| Primer 28 | Alkalihalobacillus | ACGGCATGGGCAATTTGC | GCCAAACCGGTTTCTTGCA | 59.73 | 59.56 | 166 | 120 |
100.00%
|
50.00%
|
| Primer 29 | Alkalihalobacillus | TGGTTGGGGAACATGGCA | AGGGCATTACGCAGAGCA | 59.06 | 59.01 | 189 | 120 |
100.00%
|
50.00%
|
| Primer 30 | Alkalihalobacillus | TTGCTGTAGGTGACCCAGT | TCCCCAGATCACCCTCAACT | 58.15 | 59.88 | 186 | 120 |
100.00%
|
50.00%
|