| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Alkalihalobacillus | CATTGCTGTAGGTGACCCAGT | TCCCCAGATCACCCTCAACT | 60.00 | 59.88 | 188 | 120 |
100.00%
|
50.00%
|
| Primer 32 | Alkalihalobacillus | ACATTGCTGTAGGTGACCCAG | TCCCCAGATCACCCTCAACT | 60.00 | 59.88 | 189 | 120 |
100.00%
|
50.00%
|
| Primer 33 | Alkalihalobacillus | AGGACATTGCTGTAGGTGACC | TCCCCAGATCACCCTCAACT | 59.72 | 59.88 | 192 | 120 |
100.00%
|
50.00%
|
| Primer 34 | Alkalihalobacillus | ATTGCTGTAGGTGACCCAGT | TCCCCAGATCACCCTCAACT | 58.64 | 59.88 | 187 | 120 |
100.00%
|
50.00%
|
| Primer 35 | Alkalihalobacillus | AGGGCAGTTACAAAATCCGGA | AGCTTGGACAATGCCTCACT | 59.65 | 59.60 | 189 | 120 |
100.00%
|
50.00%
|
| Primer 36 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TGGGCAGTTGGGAATGCA | 58.41 | 59.47 | 238 | 120 |
100.00%
|
50.00%
|
| Primer 37 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TTGGGCAGTTGGGAATGCA | 58.41 | 60.15 | 239 | 120 |
100.00%
|
50.00%
|
| Primer 38 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TTGGGCAGTTGGGAATGC | 58.41 | 58.21 | 239 | 120 |
100.00%
|
50.00%
|
| Primer 39 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TGGGCAGTTGGGAATGCAC | 58.41 | 60.91 | 238 | 120 |
100.00%
|
50.00%
|
| Primer 40 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TTTGGGCAGTTGGGAATGC | 58.41 | 58.94 | 240 | 120 |
100.00%
|
50.00%
|