| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TTTTGGGCAGTTGGGAATGC | 58.41 | 59.60 | 241 | 120 |
100.00%
|
50.00%
|
| Primer 42 | Alkalihalobacillus | TGGTTGCTGAATTTGTAGGCA | TTTGGGCAGTTGGGAATGCA | 58.41 | 60.76 | 240 | 120 |
100.00%
|
50.00%
|
| Primer 43 | Alkalihalobacillus | TTTGTTGCCACCGCTTGC | GGACTTGTCCGCTTGCAAC | 59.89 | 59.72 | 244 | 120 |
100.00%
|
50.00%
|
| Primer 44 | Alkalihalobacillus | AGCGGTCAACATTGCGGA | GTCCGCACTCCGCTCTAAA | 59.97 | 59.79 | 298 | 119 |
100.00%
|
50.00%
|
| Primer 45 | Alkalihalobacillus | ACAGCTCGTTTCCGCACA | GGCTGGTGCATAGTACGCA | 59.89 | 60.15 | 246 | 119 |
100.00%
|
50.00%
|
| Primer 46 | Alkalihalobacillus | AGGTTTTCTTCGGAAGGGCA | AAACGACTTGCCCTCCAAAA | 59.52 | 58.23 | 202 | 119 |
100.00%
|
50.00%
|
| Primer 47 | Alkalihalobacillus | ACGGATGCTCTTCACCAACA | AGTTCGCCTCTTCTGAAGCA | 59.60 | 59.32 | 199 | 119 |
100.00%
|
50.00%
|
| Primer 48 | Alkalihalobacillus | TCATTACGCGAGGCAGAGC | ACCTGTACGAGCCGTTGTG | 60.23 | 60.01 | 243 | 118 |
100.00%
|
50.00%
|
| Primer 49 | Alkalihalobacillus | GCTCAATTGAACGATCGGGTG | CGAACAAGTCCACCTGAAGC | 59.94 | 59.13 | 166 | 118 |
100.00%
|
50.00%
|
| Primer 50 | Alkalihalobacillus | TGCTTGGGGTTTGGCTGT | ATCAGTCGTTGCACGCCA | 59.72 | 59.97 | 214 | 117 |
100.00%
|
50.00%
|