| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Brachyspira | GCGTTCCTTGCCCAAAACC | TCGTTTCCCTGTCCCATAGC | 60.30 | 59.46 | 271 | 145 |
100.00%
|
66.67%
|
| Primer 2 | Brachyspira | GCGTTCCTTGCCCAAAACC | ATCGTTTCCCTGTCCCATAGC | 60.30 | 59.86 | 272 | 145 |
100.00%
|
66.67%
|
| Primer 3 | Brachyspira | AGCGTCTTGAAGTTTGCCG | TGCTTTATCCACCAAACACCA | 59.06 | 58.05 | 289 | 135 |
100.00%
|
133.33%
|
| Primer 4 | Brachyspira | CAGCGTCTTGAAGTTTGCCG | TGCTTTATCCACCAAACACCA | 60.38 | 58.05 | 290 | 135 |
100.00%
|
133.33%
|
| Primer 5 | Brachyspira | AGAATGCGGTGCTTGTGC | AACCGCATTGCACAGCAC | 59.04 | 59.66 | 173 | 132 |
100.00%
|
100.00%
|
| Primer 6 | Brachyspira | TGCTGATGACGGTACTACTGG | AACCAACCGCTAGCTCCTG | 59.25 | 59.70 | 219 | 131 |
100.00%
|
100.00%
|
| Primer 7 | Brachyspira | AACGGACTTGAAATGCCCAT | ATGGCATCACCCACAGCT | 58.08 | 58.91 | 155 | 128 |
100.00%
|
66.67%
|
| Primer 8 | Brachyspira | AACGGACTTGAAATGCCCAT | TATGGCATCACCCACAGCT | 58.08 | 58.69 | 156 | 128 |
100.00%
|
66.67%
|
| Primer 9 | Brachyspira | AACGGACTTGAAATGCCCAT | TGGCATCACCCACAGCT | 58.08 | 58.38 | 154 | 128 |
100.00%
|
66.67%
|
| Primer 10 | Brachyspira | GCGTCTTGAAGTTTGCCGAA | TGCTTTATCCACCAAACACCA | 59.69 | 58.05 | 288 | 128 |
100.00%
|
100.00%
|