| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Brachyspira | AACGGACTTGAAATGCCCAT | TGGCATCACCCACAGCTTTT | 58.08 | 60.47 | 154 | 120 |
100.00%
|
66.67%
|
| Primer 22 | Brachyspira | AGGTGCTAGAGTATCAGGACC | GCTGAACATCAACCCCAGC | 58.05 | 59.11 | 203 | 120 |
100.00%
|
66.67%
|
| Primer 23 | Brachyspira | TTGCTTGCCCTGCTTCTCC | CAGCTTCTCACTCCGTCTGG | 60.60 | 60.11 | 214 | 120 |
100.00%
|
66.67%
|
| Primer 24 | Brachyspira | GGAGAATGCGGTGCTTGTG | AACCGCATTGCACAGCAC | 59.50 | 59.66 | 175 | 119 |
100.00%
|
100.00%
|
| Primer 25 | Brachyspira | GGTGCTGGTATTGCTGTTGG | AGCACCTAAAACACCGCTG | 59.47 | 58.37 | 195 | 118 |
100.00%
|
133.33%
|
| Primer 26 | Brachyspira | CCAAGCAGATTCGGTGTTGC | TTCACCGCAGTCATACCAGC | 60.11 | 60.39 | 270 | 118 |
100.00%
|
66.67%
|
| Primer 27 | Brachyspira | GAGAATGCGGTGCTTGTGC | AACCGCATTGCACAGCAC | 60.15 | 59.66 | 174 | 118 |
100.00%
|
100.00%
|
| Primer 28 | Brachyspira | GCGTTCCTTGCCCAAAACC | TCATCGTTTCCCTGTCCCAT | 60.30 | 58.71 | 274 | 118 |
100.00%
|
100.00%
|
| Primer 29 | Brachyspira | TGGAGAACAGAGCGGAGAAC | ACCACCTTTGTCAACCAACAT | 59.40 | 58.25 | 187 | 118 |
100.00%
|
66.67%
|
| Primer 30 | Brachyspira | GCGGTTTACGTCAGGCTGA | AAGCAACAGGAGCACCACC | 60.37 | 60.53 | 285 | 110 |
100.00%
|
66.67%
|