| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Brachyspira | GCGGTTACATGGGAGAGGAT | CCTCTTCAACGCTGTCAAGC | 59.24 | 59.49 | 183 | 110 |
100.00%
|
50.00%
|
| Primer 32 | Brachyspira | TGGTGAGCCTGTATGCGT | CGCCATGTCCTGTAAGCGA | 58.61 | 60.15 | 260 | 109 |
100.00%
|
50.00%
|
| Primer 33 | Brachyspira | GCGTCTTGAAGTTTGCCGA | TGCTTTATCCACCAAACACCA | 59.06 | 58.05 | 288 | 109 |
100.00%
|
100.00%
|
| Primer 34 | Brachyspira | AGCGTCTTGAAGTTTGCCGA | TGCTTTATCCACCAAACACCA | 60.53 | 58.05 | 289 | 109 |
100.00%
|
100.00%
|
| Primer 35 | Brachyspira | GCGTTCCTTGCCCAAAACC | AGTTCATCGTTTCCCTGTCCC | 60.30 | 60.00 | 277 | 109 |
100.00%
|
75.00%
|
| Primer 36 | Brachyspira | GCGTTCCTTGCCCAAAACC | GTTCATCGTTTCCCTGTCCC | 60.30 | 58.55 | 276 | 109 |
100.00%
|
75.00%
|
| Primer 37 | Brachyspira | TGGGCAAAACAGTATGGCAT | GGCTCGGCAAACTTAATACCC | 58.06 | 59.33 | 236 | 109 |
100.00%
|
50.00%
|
| Primer 38 | Brachyspira | GCGGTTACATGGGAGAGGA | CCTCTTCAACGCTGTCAAGC | 58.79 | 59.49 | 183 | 109 |
100.00%
|
50.00%
|
| Primer 39 | Brachyspira | GCTGCTTGGAAGGCAATGG | TCTGCCAAAGGTCCCCAAG | 59.78 | 59.54 | 221 | 107 |
100.00%
|
50.00%
|
| Primer 40 | Brachyspira | TGATGCTCCTGGACTTGGC | GCCGTAGCTTCAGCATGGA | 59.70 | 60.15 | 186 | 107 |
100.00%
|
50.00%
|