| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Brachyspira | TCGGACCTGTTACGGTTGC | ACGGCATTAACCATAGCAAGT | 60.01 | 58.00 | 244 | 107 |
100.00%
|
50.00%
|
| Primer 42 | Brachyspira | GACCTGTTACGGTTGCTGC | ACGGCATTAACCATAGCAAGT | 59.13 | 58.00 | 241 | 107 |
100.00%
|
50.00%
|
| Primer 43 | Brachyspira | CCTGTTACGGTTGCTGCTG | ACGGCATTAACCATAGCAAGT | 59.13 | 58.00 | 239 | 107 |
100.00%
|
50.00%
|
| Primer 44 | Brachyspira | AGACGCTGATGCCATGCA | TGCGGTACACGTCATCATTGT | 59.73 | 60.34 | 197 | 107 |
100.00%
|
50.00%
|
| Primer 45 | Brachyspira | AGACGCTGATGCCATGCA | TGCGGTACACGTCATCATTG | 59.73 | 58.64 | 197 | 107 |
100.00%
|
50.00%
|
| Primer 46 | Brachyspira | AGACGCTGATGCCATGCA | GCGGTACACGTCATCATTGT | 59.73 | 58.64 | 196 | 107 |
100.00%
|
50.00%
|
| Primer 47 | Brachyspira | AGACGCTGATGCCATGCA | TTGCGGTACACGTCATCATTG | 59.73 | 59.27 | 198 | 107 |
100.00%
|
50.00%
|
| Primer 48 | Brachyspira | ATGGGCAAAACAGTATGGCA | GGCTCGGCAAACTTAATACCC | 58.06 | 59.33 | 237 | 107 |
100.00%
|
50.00%
|
| Primer 49 | Brachyspira | TGCGGTTCTTCAAGAGAGCA | ACTCAGGAAAAGGCTCTGCT | 59.61 | 58.94 | 244 | 106 |
100.00%
|
100.00%
|
| Primer 50 | Brachyspira | TGCCAGCGGAGAATTAGCT | CTCCTCTTCCGGATCTGCA | 59.09 | 58.49 | 229 | 106 |
100.00%
|
50.00%
|