| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 91 | Brachyspira | TTCAGCGGAAATGAGGCTCA | ACACCAGCACCAACACCT | 59.68 | 58.99 | 260 | 99 |
100.00%
|
75.00%
|
| Primer 92 | Brachyspira | AGGAGAATGCGGTGCTTGT | AACCGCATTGCACAGCAC | 59.62 | 59.66 | 176 | 98 |
100.00%
|
75.00%
|
| Primer 93 | Brachyspira | AAGGCTGCTGATGAGGCTC | GCCCTCATCATAGCCTTTAGC | 59.78 | 58.57 | 228 | 98 |
100.00%
|
50.00%
|
| Primer 94 | Brachyspira | GCTGCTTGGAAGGCAATGG | TCTGCCAAAGGTCCCCAA | 59.78 | 58.32 | 221 | 97 |
100.00%
|
50.00%
|
| Primer 95 | Brachyspira | CAGGCGATTTAAGCGGAAGAC | AGCACTTCCAGCAACCATGT | 59.67 | 60.18 | 152 | 97 |
100.00%
|
50.00%
|
| Primer 96 | Brachyspira | GCGTTCCTTGCCCAAAACC | CGTTTCCCTGTCCCATAGCT | 60.30 | 59.46 | 270 | 97 |
100.00%
|
50.00%
|
| Primer 97 | Brachyspira | TGAGCCCACTAATGGTGTTGA | TCCATCTGGATGCTTGTGCT | 59.30 | 59.38 | 226 | 97 |
100.00%
|
50.00%
|
| Primer 98 | Brachyspira | TCCTACAGCAGAACCTCCTGT | TGAGCCATTTCCCTACCCC | 60.20 | 58.68 | 240 | 96 |
100.00%
|
50.00%
|
| Primer 99 | Brachyspira | ACAGGCGATTTAAGCGGAAG | AGCACTTCCAGCAACCATGT | 58.63 | 60.18 | 153 | 96 |
100.00%
|
50.00%
|
| Primer 100 | Brachyspira | GAAATAGGCTGCGGAAATGGA | TGGCCAAGGATCTGGAAAGT | 58.70 | 58.92 | 240 | 96 |
100.00%
|
50.00%
|