| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 111 | Brachyspira | GCTGCTTGGAAGGCAATGG | CTGCCAAAGGTCCCCAAGA | 59.78 | 59.54 | 220 | 92 |
100.00%
|
50.00%
|
| Primer 112 | Brachyspira | GCTGCTTGGAAGGCAATGG | TGCCAAAGGTCCCCAAGA | 59.78 | 58.32 | 219 | 92 |
100.00%
|
50.00%
|
| Primer 113 | Brachyspira | TTCCAGGTGCTAACTGCGG | CGCCAGCCATAACAGCAGA | 60.00 | 60.45 | 267 | 92 |
100.00%
|
100.00%
|
| Primer 114 | Brachyspira | ATGCGGTTTCCCAGGTTGT | CGCCAGCCATAACAGCAGA | 59.85 | 60.45 | 245 | 92 |
100.00%
|
50.00%
|
| Primer 115 | Brachyspira | TGCTAACTGCGGAGGATGC | CGCCAGCCATAACAGCAGA | 60.15 | 60.45 | 260 | 92 |
100.00%
|
100.00%
|
| Primer 116 | Brachyspira | ACAGGCGATTTAAGCGGAAGA | AGCACTTCCAGCAACCATGT | 60.07 | 60.18 | 153 | 92 |
100.00%
|
50.00%
|
| Primer 117 | Brachyspira | TGGTTCGGAATAGGGGAGGT | CCCAGCCTACACCAGCAAA | 59.96 | 59.93 | 277 | 92 |
100.00%
|
50.00%
|
| Primer 118 | Brachyspira | CGGACCTGTTACGGTTGCT | ACGGCATTAACCATAGCAAGT | 60.01 | 58.00 | 243 | 92 |
100.00%
|
50.00%
|
| Primer 119 | Brachyspira | ACCTGTTACGGTTGCTGCT | ACGGCATTAACCATAGCAAGT | 59.55 | 58.00 | 240 | 92 |
100.00%
|
50.00%
|
| Primer 120 | Brachyspira | TTCGGCTGAGGCTGTTTGC | GCAGGCAAAGTCATAACTCCG | 61.26 | 59.60 | 209 | 92 |
100.00%
|
75.00%
|