| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Brachyspira | ACGCATTTGGTAAGCCCTGA | AGGCTCATCAGGATCTGGAGT | 59.96 | 60.06 | 267 | 96 |
100.00%
|
50.00%
|
| Primer 102 | Brachyspira | TTGCTTGCCCTGCTTCTCC | AGCAGCTTCTCACTCCGTC | 60.60 | 59.41 | 216 | 96 |
100.00%
|
50.00%
|
| Primer 103 | Brachyspira | GTCCGCAAAGAGAAGGGGT | ACACCTGTAGCTTCACGGC | 59.62 | 60.00 | 241 | 95 |
100.00%
|
75.00%
|
| Primer 104 | Brachyspira | TTGACAGATGTATGGGCACTG | ACGCATACTTTAGTGTCGGCA | 58.01 | 60.07 | 205 | 94 |
100.00%
|
50.00%
|
| Primer 105 | Brachyspira | TTGACAGATGTATGGGCACTG | CGCATACTTTAGTGTCGGCA | 58.01 | 58.36 | 204 | 94 |
100.00%
|
50.00%
|
| Primer 106 | Brachyspira | CCTATAGGCTGCCGTGTAACT | TGCAGCACGGAATGGCAA | 59.31 | 60.60 | 291 | 94 |
100.00%
|
50.00%
|
| Primer 107 | Brachyspira | GCGTTCCTTGCCCAAAACC | CGTTTCCCTGTCCCATAGCTT | 60.30 | 60.07 | 270 | 94 |
100.00%
|
50.00%
|
| Primer 108 | Brachyspira | ACCCAAGAACTTGCCCTGT | TGCGGACAAACAGCTACAAC | 59.07 | 59.06 | 172 | 94 |
100.00%
|
50.00%
|
| Primer 109 | Brachyspira | TGTCCGCAAAGAGAAGGGG | ACACCTGTAGCTTCACGGC | 59.63 | 60.00 | 242 | 93 |
100.00%
|
75.00%
|
| Primer 110 | Brachyspira | GGCACATAAGAAAGGTGGCG | ACAACACCATTAGCAGTTGCA | 59.55 | 58.69 | 198 | 93 |
100.00%
|
50.00%
|