| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 121 | Brachyspira | TAGAATTCGGCTGAGGCTGT | GCAGGCAAAGTCATAACTCCG | 58.80 | 59.60 | 214 | 92 |
100.00%
|
75.00%
|
| Primer 122 | Brachyspira | AGACCCTTTTGCGGATACTGT | AACAGCAGCAGCCCTTGAA | 59.37 | 60.15 | 205 | 92 |
100.00%
|
75.00%
|
| Primer 123 | Brachyspira | TGCGGAGAAGGAGAATGCG | CCGGAGTACAGAAACCGCA | 60.15 | 59.71 | 196 | 91 |
100.00%
|
50.00%
|
| Primer 124 | Brachyspira | AGGCTACGGACTTAAAGAGGC | TCATTAGTGGCGTCGTACCA | 59.52 | 58.82 | 252 | 91 |
100.00%
|
50.00%
|
| Primer 125 | Brachyspira | ACTGATGACGGCTCTGTGG | ACTCCGCATGCCATTCTCT | 59.41 | 59.09 | 188 | 91 |
100.00%
|
50.00%
|
| Primer 126 | Brachyspira | GCGGAATAGACGGCTCACA | ACTTCATCGCGTACTGGCA | 59.86 | 59.41 | 202 | 91 |
100.00%
|
50.00%
|
| Primer 127 | Brachyspira | AGACGCTGATGCCATGCA | GCGGTACACGTCATCATTGTT | 59.73 | 59.27 | 196 | 91 |
100.00%
|
50.00%
|
| Primer 128 | Brachyspira | TCCTACAGCAGAACCTCCTGT | TGAGCCATTTCCCTACCCCT | 60.20 | 60.25 | 240 | 89 |
100.00%
|
50.00%
|
| Primer 129 | Brachyspira | AGGTGCCAGCGGTATGATG | AGCCCTGCTCCCATTCTGT | 59.85 | 60.93 | 251 | 89 |
100.00%
|
50.00%
|
| Primer 130 | Brachyspira | GGTGCTAGAGTATCAGGACCT | GCTGAACATCAACCCCAGC | 58.05 | 59.11 | 202 | 89 |
100.00%
|
50.00%
|