| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 481 | Brachyspira | AGTGCTGTGCCTACGCTT | CTGCTATGCGGAGTACCCA | 59.26 | 58.88 | 251 | 63 |
100.00%
|
50.00%
|
| Primer 482 | Brachyspira | GCTGGTTCTGGAAACCCTGA | CAAATTGGTCGCCTGTAGCA | 59.89 | 58.84 | 241 | 63 |
100.00%
|
50.00%
|
| Primer 483 | Brachyspira | GCTGGTTCTGGAAACCCTGA | ACAAATTGGTCGCCTGTAGC | 59.89 | 58.83 | 242 | 63 |
100.00%
|
50.00%
|
| Primer 484 | Brachyspira | CAGGTGTTGCTGGTTCTGGA | ACAAATTGGTCGCCTGTAGC | 60.18 | 58.83 | 250 | 63 |
100.00%
|
75.00%
|
| Primer 485 | Brachyspira | AAAACGGAGAGGAAGGCGT | GCACCTGCTAGAAGGGCTT | 59.25 | 59.70 | 193 | 62 |
100.00%
|
50.00%
|
| Primer 486 | Brachyspira | GCTGCTGCTGAAGGTTTCG | GGAGCAGCTTCGCCTTCAT | 59.79 | 60.45 | 188 | 62 |
100.00%
|
50.00%
|
| Primer 487 | Brachyspira | AGGACCAGAACCTTTCAGCG | CCAACCAAGTCCCCAAGCT | 59.96 | 59.85 | 253 | 62 |
100.00%
|
75.00%
|
| Primer 488 | Brachyspira | GGTGCTTCAGGCTCTGGAAA | CTCTGCTTCATTCCTCCGCT | 60.25 | 59.82 | 260 | 61 |
100.00%
|
50.00%
|
| Primer 489 | Brachyspira | TGCGGTTCTTCAAGAGAGCA | AACTCAGGAAAAGGCTCTGCT | 59.61 | 59.58 | 245 | 61 |
100.00%
|
100.00%
|
| Primer 490 | Brachyspira | GTCCGCAAAGAGAAGGGGT | CACCTGTAGCTTCACGGCT | 59.62 | 59.71 | 240 | 61 |
100.00%
|
75.00%
|