| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Brachyspira | ACCATTGCCTACAAGCATACG | GCTGAACATCAACCCCAGC | 58.71 | 59.11 | 179 | 61 |
100.00%
|
50.00%
|
| Primer 502 | Brachyspira | ACCATTGCCTACAAGCATACG | ACATCAACCCCAGCTGGA | 58.71 | 58.08 | 174 | 61 |
100.00%
|
50.00%
|
| Primer 503 | Brachyspira | ACCATTGCCTACAAGCATACG | GAACATCAACCCCAGCTGGA | 58.71 | 59.96 | 176 | 61 |
100.00%
|
50.00%
|
| Primer 504 | Brachyspira | ACCATTGCCTACAAGCATACG | ACATCAACCCCAGCTGGAAG | 58.71 | 59.96 | 174 | 61 |
100.00%
|
50.00%
|
| Primer 505 | Brachyspira | CCTTCGGAGGCTGGTGAAA | TTCCAAGCCTTCAGTCCGC | 59.63 | 60.30 | 281 | 61 |
100.00%
|
50.00%
|
| Primer 506 | Brachyspira | GCCAGCCTTATGGAAGTCAGA | TCCCCTTCCGTAAGAGCCA | 59.79 | 59.92 | 167 | 61 |
100.00%
|
50.00%
|
| Primer 507 | Brachyspira | AGCGGAATAGACGGCTCAC | TCATCGCGTACTGGCAAACT | 59.56 | 60.04 | 200 | 61 |
100.00%
|
50.00%
|
| Primer 508 | Brachyspira | TGGGGTGCATATTGGGCAA | ACTCAACACCCACAGCACT | 59.61 | 59.08 | 200 | 61 |
100.00%
|
50.00%
|
| Primer 509 | Brachyspira | GCTTGGGAAGGCGAGAACT | TTTCAGGAGCAGCTTCGCC | 60.00 | 60.67 | 250 | 61 |
100.00%
|
50.00%
|
| Primer 510 | Brachyspira | GCGAGAGATGTCTTACGGC | ACCAGCTACACGGTTAGCC | 58.13 | 59.40 | 191 | 61 |
100.00%
|
50.00%
|