| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Brachyspira | TGCTGGCGAATCTATGGCT | TTCACCTTCCCAAGCAGCT | 59.17 | 59.15 | 193 | 61 |
100.00%
|
50.00%
|
| Primer 492 | Brachyspira | ACGGAATGTGGCAGATAGCT | AAATCAGCACCCTCCGCA | 59.17 | 59.24 | 280 | 61 |
100.00%
|
75.00%
|
| Primer 493 | Brachyspira | ACGGAATGTGGCAGATAGCT | AAAATCAGCACCCTCCGCA | 59.17 | 59.93 | 281 | 61 |
100.00%
|
75.00%
|
| Primer 494 | Brachyspira | TCAAGCGAGGCCAATGCT | GTTCCGCTTGCAATAGACACA | 59.65 | 59.20 | 200 | 61 |
100.00%
|
100.00%
|
| Primer 495 | Brachyspira | TCAAGCGAGGCCAATGCT | TCTTCAAAAGTTCCGCTTGCA | 59.65 | 58.98 | 209 | 61 |
100.00%
|
100.00%
|
| Primer 496 | Brachyspira | TCAAGCGAGGCCAATGCT | AGTTCCGCTTGCAATAGACAC | 59.65 | 58.92 | 201 | 61 |
100.00%
|
100.00%
|
| Primer 497 | Brachyspira | GCTTGGAGAGTTTAGTGCGG | ACTCTTTGTGCCTCTCCGC | 58.92 | 60.00 | 168 | 61 |
100.00%
|
75.00%
|
| Primer 498 | Brachyspira | CTGCTTCATTGGAGGGGAAGA | AGCCTGTGCCAAAGGACA | 59.72 | 59.07 | 267 | 61 |
100.00%
|
75.00%
|
| Primer 499 | Brachyspira | TCAGCATGTTTGAACGCCTT | AGACGCCTCCTTAGCTCTGT | 58.68 | 60.32 | 242 | 61 |
100.00%
|
50.00%
|
| Primer 500 | Brachyspira | TCAGCATGTTTGAACGCCTT | AGACGCCTCCTTAGCTCTG | 58.68 | 58.50 | 242 | 61 |
100.00%
|
50.00%
|