| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridioides | CATTTGGCAGTTGAAGTGTGG | TGCTTCGCGAATTGCTCC | 58.25 | 58.82 | 156 | 115 |
100.00%
|
800.00%
|
| Primer 2 | Clostridioides | CCATTTGGCAGTTGAAGTGTG | TGCTTCGCGAATTGCTCC | 58.25 | 58.82 | 157 | 106 |
100.00%
|
800.00%
|
| Primer 3 | Clostridioides | AGAATTGAGGGGAGCCATTCC | GCAGATGCAGTCCATGTTCC | 59.78 | 59.26 | 285 | 104 |
100.00%
|
900.00%
|
| Primer 4 | Clostridioides | GCGGTTATGCCATGCTACC | GAACCAAAGAAGCCAGCAGTC | 59.05 | 59.73 | 169 | 102 |
100.00%
|
800.00%
|
| Primer 5 | Clostridioides | GCGGTTATGCCATGCTACC | AACCAAAGAAGCCAGCAGTC | 59.05 | 58.68 | 168 | 102 |
100.00%
|
800.00%
|
| Primer 6 | Clostridioides | GGTAAAGCGCAAGGAAGCAG | TGCCTCATCAAACTCCTCAAC | 59.83 | 58.22 | 169 | 101 |
100.00%
|
800.00%
|
| Primer 7 | Clostridioides | GGCTCAGATGTCTAGAGGTGC | ACCTCTGTAACATCTGGCCTC | 59.93 | 59.17 | 151 | 99 |
100.00%
|
800.00%
|
| Primer 8 | Clostridioides | ATGGAGAGGAAGGGAGCTCC | ACTCCCATAGGCGAATCCTC | 60.40 | 58.66 | 278 | 99 |
100.00%
|
800.00%
|
| Primer 9 | Clostridioides | ACCATTTGGCAGTTGAAGTGT | TGCTTCGCGAATTGCTCC | 58.61 | 58.82 | 158 | 98 |
100.00%
|
800.00%
|
| Primer 10 | Clostridioides | AGAAGAAGAGTGGGCAGTGG | CCCAGTTATAGGGTCTGCTGC | 59.31 | 60.20 | 235 | 96 |
100.00%
|
900.00%
|