| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Desulfovibrio | ACCACCGATACCTGGGACA | AACTGCATGCGACCCGAA | 59.92 | 59.97 | 277 | 61 |
100.00%
|
61.54%
|
| Primer 42 | Desulfovibrio | TGCCAAGCAGAGCAAGCT | AAAATTGGTGGGCACGGC | 59.89 | 59.26 | 154 | 61 |
100.00%
|
53.85%
|
| Primer 43 | Desulfovibrio | TGCAAGGTGGGCATGGAA | AGTGCAGGGTCAGCAGTTC | 59.47 | 59.93 | 172 | 61 |
100.00%
|
30.77%
|
| Primer 44 | Desulfovibrio | CCGCAAGAAAGTCAACGGC | CGCTCTTGTTGCAGTGCA | 60.08 | 58.98 | 167 | 60 |
100.00%
|
23.08%
|
| Primer 45 | Desulfovibrio | CGCAAGATCCGTATCCGCA | CGCTCTTGTTGCAGTGCA | 60.30 | 58.98 | 181 | 60 |
100.00%
|
38.46%
|
| Primer 46 | Desulfovibrio | AACTGCCCTGTGGACAACC | TTGAGCAGCACGTCAGCA | 60.15 | 59.89 | 290 | 60 |
100.00%
|
23.08%
|
| Primer 47 | Desulfovibrio | GCCTTCACCGTGGACAAGA | TTGATGCGCACGTCCAGT | 59.93 | 59.97 | 275 | 58 |
100.00%
|
23.08%
|
| Primer 48 | Desulfovibrio | TCGTGCTGGACAACTACGC | TTGATGCGCACGTCCAGT | 60.37 | 59.97 | 250 | 58 |
100.00%
|
23.08%
|
| Primer 49 | Desulfovibrio | CACCAAGCTGCGCAACAT | GGCATTCCTGACCGTCCTT | 59.35 | 59.70 | 221 | 58 |
100.00%
|
30.77%
|
| Primer 50 | Desulfovibrio | TGCTGCTGTACAAGGGCA | TGATGCGGTCACCTTCGT | 59.16 | 58.94 | 180 | 56 |
100.00%
|
30.77%
|