| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Desulfovibrio | AAGCCTAACTCCGCTCTGC | AACGGCTCTTGCGACGAT | 59.78 | 59.74 | 214 | 97 |
100.00%
|
23.08%
|
| Primer 22 | Desulfovibrio | TGAAGCGCAAGAAGACCCC | AACGGCTCTTGCGACGAT | 60.30 | 59.74 | 297 | 96 |
100.00%
|
23.08%
|
| Primer 23 | Desulfovibrio | TGTACCGCATCGCCAGTT | TGAGCACGCAGGAGATGAC | 59.34 | 59.78 | 192 | 89 |
100.00%
|
23.08%
|
| Primer 24 | Desulfovibrio | AGCTGGTGCATCGCCTTT | ATATGGTTCCAGCGCAGGG | 59.97 | 59.85 | 170 | 82 |
100.00%
|
23.08%
|
| Primer 25 | Desulfovibrio | CACCAAGCTGCGCAACAT | TGCCCTTGTACAGCAGCA | 59.35 | 59.16 | 242 | 82 |
100.00%
|
38.46%
|
| Primer 26 | Desulfovibrio | ATGGCCACCCTGAACATGG | ACAGCTGCTTGCCTTCCA | 60.00 | 59.48 | 277 | 79 |
100.00%
|
30.77%
|
| Primer 27 | Desulfovibrio | AATGCGTGCTGCCATCCA | GTTACCTTCGATGCGCACG | 60.36 | 59.66 | 151 | 76 |
100.00%
|
30.77%
|
| Primer 28 | Desulfovibrio | AGCTGGTGCATCGCCTTT | AGGATATGGTTCCAGCGCAG | 59.97 | 59.89 | 173 | 76 |
100.00%
|
23.08%
|
| Primer 29 | Desulfovibrio | TGTGGCCAAGAACGTGCA | TGGCTGGTACGCTTGTGA | 60.12 | 58.86 | 249 | 76 |
100.00%
|
30.77%
|
| Primer 30 | Desulfovibrio | ATCGGCAAGGTGCTCAAGG | CTGCTTGCCTTCGGACTCA | 60.38 | 60.00 | 222 | 76 |
100.00%
|
23.08%
|