| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Desulfovibrio | CAAATGCGACGGCTGCAA | AACTGCACGGTCCACATGA | 59.75 | 59.55 | 252 | 119 |
100.00%
|
30.77%
|
| Primer 12 | Desulfovibrio | TGAACGTCTGGACCGTGAA | TCGCGTTCTTCCTGACAGG | 58.88 | 59.71 | 177 | 117 |
100.00%
|
30.77%
|
| Primer 13 | Desulfovibrio | AACAACGGCTCCATCGGT | ACTGCTTGCGGATCAGCA | 59.25 | 59.65 | 196 | 117 |
100.00%
|
30.77%
|
| Primer 14 | Desulfovibrio | TGCACAAGATCAGCGAGCA | AGACCGCTGCGTTTCACT | 60.00 | 59.58 | 160 | 116 |
100.00%
|
30.77%
|
| Primer 15 | Desulfovibrio | AACTCGGCTCTGCGTAAGG | AACGGCTCTTGCGACGAT | 59.78 | 59.74 | 208 | 108 |
100.00%
|
23.08%
|
| Primer 16 | Desulfovibrio | TGAAGCGCAAGAAGACCCC | AACGGCTCTTGCGACGAT | 60.30 | 59.74 | 297 | 107 |
100.00%
|
23.08%
|
| Primer 17 | Desulfovibrio | CTGGAAGAACGCTGCAAGG | TGAAGACACGGATGGCCAC | 59.13 | 60.00 | 175 | 102 |
100.00%
|
30.77%
|
| Primer 18 | Desulfovibrio | ACTTGAACGTCTGGACCGT | TCGCGTTCTTCCTGACAGG | 58.88 | 59.71 | 180 | 101 |
100.00%
|
30.77%
|
| Primer 19 | Desulfovibrio | AAGTGCTCATGCTGGCCT | TGTCGTTGTCGCAGTCCAG | 59.24 | 60.30 | 165 | 100 |
100.00%
|
30.77%
|
| Primer 20 | Desulfovibrio | TCCGCAAAGTGCTGCAGT | AGTGGGCATGCTCGATGAC | 60.20 | 60.15 | 234 | 97 |
100.00%
|
30.77%
|