| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Desulfovibrio | AACGTCTGGACCGTGAAGC | TCGCGTTCTTCCTGACAGG | 60.30 | 59.71 | 175 | 176 |
100.00%
|
30.77%
|
| Primer 2 | Desulfovibrio | CAAGCGCATCTTCATCGGC | TTTCGCCAGCAGCTCCAT | 60.01 | 59.65 | 277 | 143 |
100.00%
|
23.08%
|
| Primer 3 | Desulfovibrio | GCAAGCGCATCTTCATCGG | TTTCGCCAGCAGCTCCAT | 60.01 | 59.65 | 278 | 130 |
100.00%
|
23.08%
|
| Primer 4 | Desulfovibrio | TTCCTCATCCTGCCCTGGT | TTTCGCCAGCAGCTCCAT | 60.23 | 59.65 | 213 | 128 |
100.00%
|
23.08%
|
| Primer 5 | Desulfovibrio | AAGTGCTCATGCTGGCCT | TGTCGTTGTCGCAGTCCA | 59.24 | 59.19 | 165 | 122 |
100.00%
|
30.77%
|
| Primer 6 | Desulfovibrio | CTTGAACGTCTGGACCGTGA | TCGCGTTCTTCCTGACAGG | 59.97 | 59.71 | 179 | 121 |
100.00%
|
30.77%
|
| Primer 7 | Desulfovibrio | TTGAACGTCTGGACCGTGA | TCGCGTTCTTCCTGACAGG | 58.88 | 59.71 | 178 | 121 |
100.00%
|
30.77%
|
| Primer 8 | Desulfovibrio | CAAATGCGACGGCTGCAA | GGAACTGCACGGTCCACAT | 59.75 | 60.30 | 254 | 119 |
100.00%
|
46.15%
|
| Primer 9 | Desulfovibrio | CAAATGCGACGGCTGCAA | ATGATGGAGTCGGCAGAGC | 59.75 | 59.55 | 237 | 119 |
100.00%
|
30.77%
|
| Primer 10 | Desulfovibrio | CAAATGCGACGGCTGCAA | CAGAGCGCATGGGGATACA | 59.75 | 59.55 | 224 | 119 |
100.00%
|
61.54%
|