| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Desulfovibrio | TGCAAGGTGGGCATGGAA | CAGTGCAGGGTCAGCAGTT | 59.47 | 60.23 | 173 | 55 |
100.00%
|
38.46%
|
| Primer 52 | Desulfovibrio | TGCAAGGTGGGCATGGAA | AGTGCAGGGTCAGCAGTT | 59.47 | 58.76 | 172 | 55 |
100.00%
|
23.08%
|
| Primer 53 | Desulfovibrio | AACGTCTGGACCGTGAAGC | GCTTTCGCGTTCTTCCTGAC | 60.30 | 59.84 | 179 | 52 |
100.00%
|
30.77%
|
| Primer 54 | Desulfovibrio | TGCTGACGTGCTGCTCAA | CCCGTGTGGATGTTGTGGA | 59.89 | 59.93 | 225 | 52 |
100.00%
|
23.08%
|
| Primer 55 | Desulfovibrio | TGGTCGCGTCATGCTGAA | GCTTCGAGCTTCTTGCCAC | 59.66 | 59.50 | 216 | 50 |
100.00%
|
23.08%
|
| Primer 56 | Desulfovibrio | TCCGCAACGCACATTTGG | AATGCAAATGCCGAGGCC | 59.36 | 59.11 | 260 | 49 |
100.00%
|
53.85%
|
| Primer 57 | Desulfovibrio | TGCCACGGCTTTGACGAA | TCCAGAGCAAAGTCCAGCG | 60.20 | 60.00 | 296 | 46 |
100.00%
|
30.77%
|
| Primer 58 | Desulfovibrio | AAGGCTGCCAAGAAGTCCG | AGTTGCCGAGCTGCATGT | 60.30 | 59.97 | 268 | 35 |
100.00%
|
38.46%
|
| Primer 59 | Desulfovibrio | GCAATTGCGCTATGCCGA | AAGCAGACGATGGTCAGGC | 59.28 | 60.08 | 267 | 31 |
100.00%
|
23.08%
|
| Primer 60 | Desulfovibrio | GCTGGTGCATCGCCTTTT | ATATGGTTCCAGCGCAGGG | 59.04 | 59.85 | 169 | 26 |
100.00%
|
23.08%
|