| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Megasphaera | AATCCGCTTGGACCGCAT | GGGCATACAGGCATTGGGA | 59.73 | 59.77 | 212 | 140 |
100.00%
|
66.67%
|
| Primer 2 | Megasphaera | AGCGTCTTTGAAGTCGGCT | ACCGAATGCGTGACGACA | 59.63 | 59.66 | 152 | 128 |
100.00%
|
66.67%
|
| Primer 3 | Megasphaera | AACGGCTATCGCACTCAGG | AACCCGTGCCAAAGAGCA | 59.86 | 59.81 | 208 | 127 |
100.00%
|
66.67%
|
| Primer 4 | Megasphaera | GCAGCAATGCTCGACATCC | ACAGCGATCCCCAAACCAC | 59.65 | 60.30 | 209 | 125 |
100.00%
|
66.67%
|
| Primer 5 | Megasphaera | AGCGCTGCCGAAATGGAT | TACGCCTGCGCTTTCAGT | 60.12 | 59.66 | 173 | 119 |
100.00%
|
66.67%
|
| Primer 6 | Megasphaera | AATCCGCTTGGACCGCAT | TGGGCATACAGGCATTGGG | 59.73 | 60.08 | 213 | 115 |
100.00%
|
66.67%
|
| Primer 7 | Megasphaera | TGCCTCATTTCCCTGAGCG | ATGTTGCGGACGATGCCT | 60.08 | 59.73 | 197 | 111 |
100.00%
|
66.67%
|
| Primer 8 | Megasphaera | GTGTCCAGGAAGGCGACAA | GTGAAAGGCTGACGATGGC | 59.93 | 59.20 | 244 | 108 |
100.00%
|
66.67%
|
| Primer 9 | Megasphaera | AAAGCAGACCGCATCGCT | CGGTTTTGCAGCAGCGAT | 60.05 | 59.44 | 168 | 106 |
100.00%
|
66.67%
|
| Primer 10 | Megasphaera | AAAGCAGACCGCATCGCT | TTCGTTCAGAGCCTGCTGG | 60.05 | 60.00 | 249 | 106 |
100.00%
|
66.67%
|