| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1211 | Methanosphaera | AGCACTACTATTTGCAGCCGA | CTGGTCGTGGTGTATGTTTGT | 59.79 | 58.51 | 179 | 65 |
100.00%
|
66.67%
|
| Primer 1212 | Methanosphaera | GCACTACTATTTGCAGCCGA | CTGGTCGTGGTGTATGTTTGT | 58.35 | 58.51 | 178 | 65 |
100.00%
|
66.67%
|
| Primer 1213 | Methanosphaera | CGGGAGCTACTATTGGACGA | CCCTCAGGTGCTGGTTTTG | 58.68 | 58.66 | 262 | 65 |
100.00%
|
66.67%
|
| Primer 1214 | Methanosphaera | ACCACCAACATCCCCTATTGA | AGCAGCTGCAACACCAGT | 58.72 | 59.81 | 151 | 65 |
100.00%
|
66.67%
|
| Primer 1215 | Methanosphaera | ACCACCAACATCCCCTATTGA | AGCAGCTGCAACACCAGTA | 58.72 | 59.55 | 151 | 65 |
100.00%
|
66.67%
|
| Primer 1216 | Methanosphaera | ACTGCTTTGTCCTGGCTTTT | AGAACAATGCCCAACCAAAGA | 58.22 | 58.32 | 176 | 64 |
100.00%
|
33.33%
|
| Primer 1217 | Methanosphaera | TACTGCTTTGTCCTGGCTTTT | AGAACAATGCCCAACCAAAGA | 58.06 | 58.32 | 177 | 64 |
100.00%
|
33.33%
|
| Primer 1218 | Methanosphaera | TTACTGCTTTGTCCTGGCTTT | AGAACAATGCCCAACCAAAGA | 58.06 | 58.32 | 178 | 64 |
100.00%
|
33.33%
|
| Primer 1219 | Methanosphaera | AGGCGAAGTCACTGACGAA | TCGTGGAGGGTGTGCTTT | 58.96 | 58.76 | 259 | 64 |
100.00%
|
33.33%
|
| Primer 1220 | Methanosphaera | GGCACTTGGGGAAATGGAAG | TGCTGCCCTTCGTGCTTT | 59.10 | 60.20 | 157 | 64 |
100.00%
|
66.67%
|