| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Methanosphaera | TGGAACAACCGCAAGTTCAA | CGTTTTGCTTCAACTCCAGGA | 58.54 | 59.05 | 174 | 63 |
100.00%
|
33.33%
|
| Primer 1222 | Methanosphaera | TGGAACAACCGCAAGTTCAA | TCGTTTTGCTTCAACTCCAGG | 58.54 | 59.05 | 175 | 63 |
100.00%
|
33.33%
|
| Primer 1223 | Methanosphaera | GGACCACATGCATGTACTGG | TGCCCTCAGCTTCCACAA | 58.62 | 58.75 | 184 | 63 |
100.00%
|
33.33%
|
| Primer 1224 | Methanosphaera | TGCAACCTGTTCAGGAGCA | ATTGGTACAGCGTGTCCGG | 59.47 | 60.08 | 282 | 63 |
100.00%
|
33.33%
|
| Primer 1225 | Methanosphaera | GGGGAAACAACAACACCGG | TGTGCAAGTGTACTGTCAGGA | 59.26 | 59.24 | 158 | 63 |
100.00%
|
66.67%
|
| Primer 1226 | Methanosphaera | GGGGAAACAACAACACCGG | CCACTTATTTGACGACGTGCA | 59.26 | 59.20 | 239 | 63 |
100.00%
|
66.67%
|
| Primer 1227 | Methanosphaera | GGGGAAACAACAACACCGG | TCCACTTATTTGACGACGTGC | 59.26 | 58.94 | 240 | 63 |
100.00%
|
66.67%
|
| Primer 1228 | Methanosphaera | AGCAACTGCCATAGCCCAA | CCTGTATGACATGCTCCACC | 59.62 | 58.04 | 246 | 63 |
100.00%
|
66.67%
|
| Primer 1229 | Methanosphaera | TGGCACGAGGAGCAACATA | AACCGGTAAGATAAGCAGCAG | 59.02 | 58.09 | 258 | 63 |
100.00%
|
66.67%
|
| Primer 1230 | Methanosphaera | TTGGTGATGATGCAGCGG | GTCCACCCATTGCAGCAA | 58.09 | 58.22 | 156 | 63 |
100.00%
|
66.67%
|