| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1231 | Methanosphaera | AGTAACGGGTGGAGCAGGA | CCTCACCATTCCAACGACC | 60.23 | 58.15 | 221 | 62 |
100.00%
|
33.33%
|
| Primer 1232 | Methanosphaera | TGCATGCTTGGTGGAGGA | CGCCTGCAACAATCAATGGA | 58.83 | 59.47 | 226 | 62 |
100.00%
|
66.67%
|
| Primer 1233 | Methanosphaera | AATCCATGGTGGGCAGGA | CCACCCACCATTGGTACTCC | 58.15 | 60.03 | 179 | 62 |
100.00%
|
66.67%
|
| Primer 1234 | Methanosphaera | ACCTGAATGTTCACCATGGC | AGCTCTTCGGGATTGGGGA | 58.45 | 60.31 | 181 | 62 |
100.00%
|
66.67%
|
| Primer 1235 | Methanosphaera | TGTGGAGCATGTGGTCTTGT | CGCCAATCAATCATGGTGGA | 59.53 | 58.61 | 158 | 62 |
100.00%
|
66.67%
|
| Primer 1236 | Methanosphaera | ATCGTTGGAACAGGCGAAGT | GTGTGTTGTTTGTTGCAGCT | 59.97 | 58.01 | 202 | 61 |
100.00%
|
33.33%
|
| Primer 1237 | Methanosphaera | AGCAGGAGAAACAGCAGCA | GCTATTGCTCCTTCAGGGGT | 59.55 | 59.45 | 186 | 61 |
100.00%
|
33.33%
|
| Primer 1238 | Methanosphaera | AGCAGCAGGAGAAACAGCA | GCTATTGCTCCTTCAGGGGT | 59.55 | 59.45 | 189 | 61 |
100.00%
|
33.33%
|
| Primer 1239 | Methanosphaera | GCAGGAGAAACAGCAGCAG | GCTATTGCTCCTTCAGGGGT | 59.13 | 59.45 | 185 | 61 |
100.00%
|
33.33%
|
| Primer 1240 | Methanosphaera | CCTTCATGGGCTTTTCAGGAA | GCAAGTTTCAGATTTCCTGGC | 58.47 | 58.05 | 185 | 61 |
100.00%
|
33.33%
|