| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1261 | Methanosphaera | TTCCTGGACCAAGAGGAGGA | TGCTTCACAATCCCCTGGT | 59.51 | 58.84 | 176 | 61 |
100.00%
|
66.67%
|
| Primer 1262 | Methanosphaera | ACTTGTAGGCGTTGGTGGAA | ACTTGTCATGGCAGTTCCTGA | 59.53 | 59.58 | 277 | 61 |
100.00%
|
66.67%
|
| Primer 1263 | Methanosphaera | ACTTGTAGGCGTTGGTGGAA | GTCATGGCAGTTCCTGAAACC | 59.53 | 59.46 | 273 | 61 |
100.00%
|
66.67%
|
| Primer 1264 | Methanosphaera | AAGGGAAAAGAGAAGTGGGGT | TTGATACCTCCTGAAGCGGA | 58.86 | 58.14 | 224 | 61 |
100.00%
|
66.67%
|
| Primer 1265 | Methanosphaera | AAGGGAAAAGAGAAGTGGGGT | CCTCCTGAAGCGGATTTTCTC | 58.86 | 58.72 | 218 | 61 |
100.00%
|
66.67%
|
| Primer 1266 | Methanosphaera | GGAGGGGTGGTTACTCTTTGG | TTCCAAGTCCACAACCTGCT | 60.00 | 59.45 | 229 | 61 |
100.00%
|
66.67%
|
| Primer 1267 | Methanosphaera | TGCTCCAACGGGTTTAGGT | TCCAACATCCACCAACACCAA | 58.85 | 60.06 | 269 | 61 |
100.00%
|
66.67%
|
| Primer 1268 | Methanosphaera | GCTGGAGTAGGTGACATGGT | TGGCCAAAGATCTGCAGCT | 59.09 | 59.62 | 228 | 61 |
100.00%
|
66.67%
|
| Primer 1269 | Methanosphaera | AGACTTGCTTCAGCTGGAGT | TGGCCAAAGATCTGCAGCT | 58.94 | 59.62 | 240 | 61 |
100.00%
|
66.67%
|
| Primer 1270 | Methanosphaera | AGGCGACATCACCGAGGAA | TGCTCCTCTACCGGAACCA | 60.98 | 59.92 | 175 | 61 |
100.00%
|
66.67%
|