| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1281 | Methanosphaera | GTCGTGATTGGGCAGCAGT | CTGCAGCAATCACATGTGGT | 60.67 | 59.11 | 216 | 61 |
100.00%
|
66.67%
|
| Primer 1282 | Methanosphaera | GTTCGTCGTGATTGGGCAG | CTGCAGCAATCACATGTGGT | 59.21 | 59.11 | 220 | 61 |
100.00%
|
66.67%
|
| Primer 1283 | Methanosphaera | AGACAACCATTCCCAGGGC | AATGGCCATGGAATGTCTGGT | 59.62 | 59.99 | 278 | 61 |
100.00%
|
66.67%
|
| Primer 1284 | Methanosphaera | ACTCTGTTGTCGATGCACAAT | CCCCAGGTGTTGAAACTTCC | 58.23 | 58.67 | 156 | 61 |
100.00%
|
66.67%
|
| Primer 1285 | Methanosphaera | ATGGCTGCTGTTGCTGGAA | TGAGGAGGTGCATATGCCG | 60.23 | 59.55 | 216 | 61 |
100.00%
|
66.67%
|
| Primer 1286 | Methanosphaera | AGGTGTGGCATTGGGTGAA | CAGCCCATGTTACTGCTTCAA | 59.46 | 58.84 | 248 | 61 |
100.00%
|
66.67%
|
| Primer 1287 | Methanosphaera | AGGTGTGGCATTGGGTGAA | AGGATATACTGCAGCCCATGT | 59.46 | 58.66 | 259 | 61 |
100.00%
|
66.67%
|
| Primer 1288 | Methanosphaera | TTGGAAGCGGACCACTACC | CGCGTTCCCATACATTTGCA | 59.32 | 59.55 | 252 | 60 |
100.00%
|
33.33%
|
| Primer 1289 | Methanosphaera | GACGTAACCACCCCAAGCA | TCCTCCATCATCTGCAAGTGC | 59.93 | 60.41 | 183 | 60 |
100.00%
|
33.33%
|
| Primer 1290 | Methanosphaera | CGCCAACAGCAGATATGCA | AGCATCTCCACGTGTAGCAC | 58.60 | 60.11 | 284 | 60 |
100.00%
|
33.33%
|